毕赤酵母重组菌GS115-Ch-Glu发酵培养基设计及发酵条件优化  

Designing the Medium for Pichia pastoris GS115-Ch-Glu Fermentation and Optimizing Its Fermentation Conditions

作　　者：冯爱娟[1,2] 吴酬飞[3] 王江海[3] 邓毛程[1,2] 

机构地区：[1]广东轻工职业技术学院食品与生物工程系,广州510300 [2]广东高校特色调味品工程技术开发中心,广州510300 [3]中山大学海洋学院,广州510006

出　　处：《湖北农业科学》2015年第5期1155-1158,共4页Hubei Agricultural Sciences

基　　金：广东高校特色调味品工程技术开发中心建设项目(GCZX-B1103);广东轻工职业技术学院校级项目(KJ201107)

摘　　要：以毕赤酵母重组菌(Pichia pastoris)GS115-Ch-Glu为研究对象,进行了亚麻子发酵脱毒工艺的研究,并对重组菌的发酵培养条件进行优化。结果表明,毕赤酵母重组菌GS115-Ch-Glu发酵扩大培养的培养基最佳碳源为2.5%玉米淀粉,最佳氮源为5.0%黄豆粕。优化后的基础发酵培养基发酵条件为温度28℃、转速250 r/min、发酵时间33 h、初始p H 6.5、接菌量1.5%、装液量50 m L/250 m L。在此最佳发酵条件下,菌体产量为9.0×1010个/m L。Pichia pastoris GS115-Ch-Glu as a sort of useful recombinant yeast was used to detoxify in linseed. Its fermentation conditions were optimized. The results showed that the optimal carbon and nitrogen source were 2.5% corn starch and 5.0% soybean meal. The optimal fermentation conditions in basal medium were the temperature of 28 ℃, the speed of 250 r/min, the incubation time of 33 hours, the initial pH value of 6.5, the inoculation rate of 1.5% and the liquid volume of 50 mL/250 mL. Under these conditions, the fermentation yield of the cell production was per milliliter of 9.0× 10^10 strains.

关 键 词：毕赤酵母(Pichia pastoris) 发酵条件 优化 

分 类 号：Q815[生物学—生物工程]