油橄榄叶不同溶剂超声提取物的抑菌和抗氧化活性及其主要成分分析  

Assessment of Main Phenolics, Antimicrobial and Antioxidant Activities of Different Solvents Extracts by Ultrasound-assisted Method from Olive Leaves

作　　者：吴遵秋 姜友军[1] 陈华萍[1] 杨泽身 黄乾明[1] 

机构地区：[1]四川农业大学理学院,雅安625014 [2]凉山州中泽新技术开发有限责任公司,西昌615000

出　　处：《天然产物研究与开发》2014年第A02期197-204,共8页Natural Product Research and Development

基　　金：四川省科技厅科技支撑项目（12ZC2220）

摘　　要：采用不同溶剂对油橄榄叶进行超声波辅助提取，分析各溶剂提取物的抑菌、抗氧化活性和主要成分。结果显示：各溶剂提取物的抑菌与抗氧化活性强弱顺序基本一致，80％甲醇提取物的抑菌和抗氧化活性最强，其次为80％乙醇提取物，乙酸乙酯提取物的抗氧化活性最弱，而水提取物的抑菌活性最弱；不同溶剂提取物等比例混合物的抑菌活性无明显增强。LC-MS分析显示：五种溶剂提取物共得九种主要成分，水和醇提取的成分较多，丙酮和乙酸乙酯提取的成分较少，各溶剂提取物均含橄榄苦苷和Ligstroside两种成分，水提取物和醇提取物中各特有两种主要成分。The dry powder of olive leaves were extracted by ultrasonic assisted with five solvents to obtain different com= ponents, and analyzed main phenolics, antimicrobial and antioxidant activities of components. The results showed that there was a similar sequence to antibacterial and antioxldant activities of different solvent extracts. The 80% methanol extract was the strongest on antibacterial and antioxidant actlvities,followed by 80% ethanol extract. Antionidant activities of the ethyl acetate extract was the weakest, antibacterial activities of the water extract was the weakest. There were not significant enhancement for antibacterial activities from the mixtures of different components. LC-MS analysis showed that five solvent extracts had a total of nine main components, these components of water, methanol and ethanol extraction had more compounds. The water extract and the alcohol extract had unique two main components, respectively.

关 键 词：油橄榄叶 不同溶剂 抑菌活性 抗氧化活性 LC-MS分析 

分 类 号：Q946.8[生物学—植物学] Q505