乌梅丸及其拆方对溃疡性结肠炎大鼠结肠黏膜组织SOD与MDA影响  

The Effect on SOD and MDA in Colonic Mucosal Tissue of Ulcerative Colitis Rat Treated by Wumei Pill and Its Disassembled Prescriptions

作　　者：李斌[1] 谷松[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847

出　　处：《辽宁中医药大学学报》2015年第5期48-50,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：辽宁省教育厅一般项目(L2012338)

摘　　要：目的:观察乌梅丸及其拆方对溃疡性结肠炎大鼠结肠黏膜组织SOD与MDA的影响,探讨乌梅丸的配伍机理,寻求溃疡性结肠炎治疗的新方法。方法:健康成年Wistar大鼠雌雄各40只,随机分为8组,即正常组、模型组、西药对照组、乌梅丸组、辛开苦降组、辛开组、苦降组、补益组。造模成功后分别给予相应治疗,并检测结肠黏膜组织SOD与MDA水平。结果:与模型组大鼠比较,各治疗组大鼠结肠黏膜组织SOD水平显著升高,MDA水平显著降低(P<0.01);西药组、乌梅丸组及辛开苦降组大鼠结肠黏膜组织SOD水平无显著性差异(P>0.05),且显著高于辛开组、苦降组及补益组(P<0.01);西药组、乌梅丸组及辛开苦降组大鼠结肠黏膜组织MDA水平无显著性差异(P>0.05),且显著低于辛开组、苦降组及补益组(P<0.01)。结论:乌梅丸可通过减轻结肠黏膜脂质过氧化反应治疗溃疡性结肠炎;辛开苦降、寒温并施及乌梅酸收柔肝的配伍方式可能是乌梅丸治疗溃疡性结肠炎的内在机制。Objective： By observing the regulating effect of Wumei Pill and its disassembled prescriptions on SOD and MDA in colonic mueosa tissue of ulcerative colitis （ UC ）rats, to investigate formulation mechanism of Wumei Pill and find out a new approach for the treatment for UC. Methods： Healthy adult wistar rats of male and female of 40 animals each were randomly divided into 8 groups, which is the normal group, model group, the western medicine group, Wumei Pill group, Xinkai Kujiang group, Xinkai group, Kujiang group and tonic group.After corresponding treatment SOD and MDA in colonic mueosa tissue was detected. Results ： Compared with the model group, SOD level of each treating group increased significantly （ P〈0.01 ）, and MDA level of each treating group decreased significantly （P〈0.01） .Each SOD level of the western medicine group, Wumei Pill group and Xinkai Kujiang group which increases significantly （P〈0.01）according to Xinkai group, Kujiang group and tonic group has no significant difference （ P〉0.05 ）. Each MDA level of the western medicine group, Wumei pill group and Xinkai Kujiang group which decreases significantly （P〈0.01）according to Xinkai group, Kujiang group and tonic group has no significant difference （ P〉0.05 ）. Conclusion ： Wumei Pill can treat UC by reducing colonic mucous membrane lipid peroxidation ; the characteristic of formulation mechanism of Wumei Pill is the coordination of pungent drugs and bitter dugs.

关 键 词：乌梅丸 拆方 溃疡性结肠炎 超氧化物歧化酶 丙二醛 

分 类 号：R574.62[医药卫生—消化系统]