茶多酚对脂多糖诱导人牙周膜成纤维细胞凋亡及炎症因子的影响  

Effect of tea polyphenols on apoptosis and inflammatory cytokines of human periodontal ligament fibroblasts induced by lipopolysaccharide

作　　者：王珺[1,2] 欧龙 罗芸[3] 谢潇[1] 李华[4] 

机构地区：[1]解放军总医院口腔科牙周黏膜病科,硕士北京100853 [2]海军机关门诊部口腔科,副主任医师北京100841 [3]解放军总医院老年病医学研究所细胞生物研究室,副研究员北京100853 [4]解放军总医院口腔科,护士长主管护师北京100853

出　　处：《中华老年口腔医学杂志》2015年第2期80-84,共5页Chinese Journal of Geriatric Dentistry

基　　金：十一五军队保健课题(项目编号:10BJZ03)

摘　　要：目的:探讨茶多酚对脂多糖(LPS)诱导人牙周膜成纤维细胞(HPDLFs)凋亡及肿瘤坏死因子-α-(TNF-α)、白介素6(IL-6)分泌的影响。方法:体外培养HPDLFs,MTT法筛选茶多酚浓度梯度,再分别用LPS(1μg/ml)和LPS(1μg/m1)+茶多酚(10μg/ml、50μg/ml、100μg/ml)刺激HPDLFs,流式细胞仪检测细胞凋亡,Elisa法检测TNF一α、IL-6分泌。结果:LPS可显著诱导HPDLFs凋亡,上调TNF一α、IL-6表达。茶多酚可显著降低LPS诱导的细胞凋亡和TNF-α、IL-6表达,且呈量效依赖性。结论:茶多酚可通过有效降低LPS诱导的细胞凋亡和TNF一α、IL-6表达。Objective：To evaluate the effect of tea polyphenols on apoptosis and expression of tumor necrosis factor-α（TNF-α）and interleukin-6 （IL-6）in LPS-induced human periodontal ligament fibroblasts（HPDLFs）.Methods：HPDLFs were cultured in vitro, and treated with LPS（1μg/ml）or LPS（1μg/ml） plus different concentrations of tea polyphenols（10μg/ml, 50μg/ml, 100μg/ml）, respectively. Apoptosis was determined by flow cytometer, and the expressions of TNF-αand IL-6 were determined by Elisa.Results：Apoptosis was markedly enhanced and the expression of TNF-αand IL-6 were significantly up-regulated in HPDLFs stimulated by LPS. Tea polyphenols significantly inhibited LPS-stimulated apoptosis, as well as reduced the expression of TNF-αand IL-6.Conclusion：Tea polyphenols can effectively reduce apoptosis and the expression of TNF-αand IL-6 in LPS-induced HPDLFs.

关 键 词：人牙周膜成纤维细胞 茶多酚 脂多糖 肿瘤坏死因子-α 白介素-6 细胞凋亡 

分 类 号：R781.4[医药卫生—口腔医学]