Gastrin,somatostatin,G and D cells of gastric ulcer in rats  

作　　者：Feng-Peng Sun Yu-Gang Song Wei Cheng Tong Zhao Yong-Li Yao,Department of Gastroenterology,Zhujiang Hospital,First Military Medical University,Guangzhou 510282,Guangdong Province,China 

出　　处：《World Journal of Gastroenterology》2002年第2期375-378,共4页世界胃肠病学杂志（英文版）

基　　金：Natural Science Foundation of Guangdong Province,China,No.010578;Important Technological Issue of Guangdong Province,No.99-13

摘　　要：AIM: To investigate the relationship among gastrin, somatostatin, G and D cells in gastric ulcer and in its healing process in rats. METHODS: Fourty-nine Wistar rats were divided into 7 groups. The gastric ulcer model was induced by acetic acid successfully. The gastrin and the somatostatin in rat plasma, gastric fluid and antral tissue were measured by radioimmunoassay(RIA). G and D cells in antral mucosa were analyzed with polyclonal antibody of gastrin and somatostatin by immunohistochemical method and Quantimet 500 image analysis system. RESULTS: In gastric ulcer, the level of gastrin in plasma, gastric fluid, and antral tissue increased, that of somatostatin declined, and the disorder gradually recovered to the normal level in the healing process. Immunohistochemical technique of G and D cells in antral mucosa demonstrated that the number of G cells increased and that of D cells decreased, both areas of G and D cells declined, the ratio of number and area of G/D increased in gastric ulcer, and the disorder gradually recovered in the healing process. CONCLUSION: In gastric ulcer, the increased gastrin secreted by G cells, the declined somatostatin secreted by D cells, and the disordered G/D cell ratio can lead to gastrointestinal dysfunction.AIM: To investigate the relationship among gastrin,somatostatin, G and D calls in gastric ulcer and in its healing process in rats.METHODS: Fourty-nine Wistar rats were divided into 7groups. The gastric ulcer model was induced by acetic acid successfully. The gastrin and the somatostatin in rat plasma, gastric fluid and antral tissue were measured by radioimmunoassay(RIA). G and D cells in antral mucosa were analyzed with polyclonal antibody of gastrin and somatostatin by immunohistochemical method and Quantimet 500 image analysis systemRESULTS: In gastric ulcer, the level of gastrin in plasma,gastric fluid, and antral tissue increased, that ofsomatostatin declined, and the disorder gradually recoveredto the normal level in the healing process.Immunohistochemical technique of G and D cells in antralmucosa demonstrated that the number of G cells increasedand that of D cells decreased, both areas of G and D cellsdeclined, the ratio of number and area of G/D increased ingastric ulcer, and the disorder gradually recovered in thehealing process.CONCLUSION: In gastric ulcer, the increased gastrinsecreted by G cells, the declined somatostatin secreted by Dcells, and the disordered G/D cell ratio can lead togastrointestinal dysfunction.

关 键 词：Animals Disease Models  Animal Gastric Mucosa Gastrin-Secreting Cells GASTRINS Male RATS Rats  Wistar Research Support  Non-U.S. Gov't SOMATOSTATIN Somatostatin-Secreting Cells Stomach Ulcer 

分 类 号：R573.1[医药卫生—消化系统]