GABA transporter 1 transcriptional starting site exhibiting tissue specific difference  

作　　者：JinXP HuangF 

机构地区：[1]InstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologicalScienceAcademyofSciences,320YueyangRoad,Shanghai200031,China [2]InstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologicalS

出　　处：《Cell Research》2001年第2期161-163,共3页细胞研究（英文版）

基　　金：foundations from Chinese Academy of Sciences and Special Funds for Major State Basic reseaxch of China (G1999053903).

摘　　要：GABA transporter 1(GAT1) takes important roles in multiple physiological processes through the uptake and release of GABA, but the regulation of GAT1 gene expression in different tissues is rarely known. To address the question, first, 5’ Rapid amplification of cDNA end (RACE) was used to determine GAT1 transcriptional starting sites in neonatal mouse cerebral cortex and intestine, adult mouse brain and adult rat testis. The products of 5’RACE were confirmed by DNA sequencing. We found that the transcript of GAT1 in neonatal mouse cerebral cortex and adult mouse brain starts at the same site (inside of exon 1), while in mouse intestine, GAT1 starts transcription in intron 1, and in rat testis, the transcript of GAT1 has an additional untranslation exon to the 5’ direction.

关 键 词：Membrane Transport Proteins Organic Anion Transporters Aging ANIMALS Animals  Newborn Base Sequence Brain Carrier Proteins DNA  Complementary EXONS GABA Plasma Membrane Transport Proteins Gene Expression Regulation INTESTINES INTRONS Male Membrane Proteins MICE Mice  Inbred BALB C Molecular Sequence Data Nucleic Acid Amplification Techniques Research Support  Non-U.S. Gov't Testis Transcription  Genetic 

分 类 号：Q26[生物学—细胞生物学]