突变型p53与Nampt在胃癌组织中的表达及其与预后的关系  

Expressions of mutant p53 and Nampt in gastric cancer tissue and their relations with prognosis

作　　者：李亮[1] 陈锐[1] 刘俊松[1] 常帅[1] 贺仕才[1] 王泽星[1] 王进[1] 车向明[1] 赵伟[1] 

机构地区：[1]西安交通大学第一附属医院普通外科,陕西西安710061

出　　处：《中国普通外科杂志》2015年第4期505-510,共6页China Journal of General Surgery

基　　金：国家自然科学基金资助项目(81172357);陕西省科技攻关资助项目(2013SF2-03)

摘　　要：目的:探讨突变型p53(mutp53)和尼克酰胺磷酸核糖转移酶(Nampt)在胃癌组织中的表达及其对患者预后的影响。方法:用免疫组化法检测68例胃癌患者胃癌组织及癌旁正常胃黏膜组织中p53(免疫组化检测到的p53主要为mutp53)与Nampt的表达,分析mutp53与Nampt表达与患者临床病理因素及预后的关系。结果:胃癌组织中mutp53与Nampt的阳性表达率均明显高于癌旁正常胃黏膜组织(均P<0.05);mutp53的高表达与肿瘤大小、浸润深度、淋巴结转移及TNM分期有关,而Nampt的高表达与肿瘤浸润深度、淋巴结转移及TNM分期有关(均P<0.05)。胃癌组织中,p53表达与Nampt表达呈明显正相关(r=0.982,P<0.05)。p53阳性表达患者的中位生存期明显短于阴性表达患者,且在p53阳性表达患者中,Nampt同时阳性患者的中位生存期明显短于Nampt阴性患者(均P<0.05)。结论:mutp53与Nampt的表达均与胃癌的恶性生物学行为相关,且两者存在一定的关联性,同时高表达患者预后差。Objective：To investigate the expressions of mutant p53（mutp53） and nicotinamide phosphoribosyltransferase（Nampt） in gastric cancer tissue and their inl uences on prognosis of the patients.Methods：Immunohistochemical staining was per formed for detection of the ex pressions of p53（immunohistochemical positive stained p53 proteins are mainly mutant type） and Nampt in specimens of gastric cancer tissues along with the adjacent normal gastric mucosa from 68 gastric cancer patients,and the relations of expression statuses of p53 and Nampt with the clinicopathologic factors and prognosis of the patients were analyzed.Results：The positive expression rates of p53 and Nampt in gastric cancer tissue were signii cantly higher than those in adjacent normal gastric mucosa（both P〈0.05）.The p53 overexpression was related to tumor size,ini ltration depth,lymph node metastasis and TNM stage,while the Nampt overexpression was associated with infiltration depth,lymph node metastasis and TNM stage（all P〈0.05）.The expression of p53 was positively correlated with that of Nampt in gastric cancer tissue（r=0.982,P〈0.05）.The median survival time in patients with p53 positive expression was signii cantly shorter than that in patients with negative p53 expression; moreover,among patients with positive p53 expression,the median survival time in those with concomitant positive Nampt expression was signii cantly shorter than in those with negative Nampt expression（both P〈0.05）.Conclusion：Both mutp53 and Nampt expressions are related to the malignant behavior of gastric cancer,further,there is certain relevance between them,and patients with both mutp53 and Nampt overexpressions may face a poor prognosis.

关 键 词：胃肿瘤 基因 p53 突变 烟酰胺核苷酸腺嘌呤转移酶 

分 类 号：R735.2[医药卫生—肿瘤]