乳扇制品中乳酸杆菌的分离鉴定及16S rRNA序列同源性分析  

Identification and 16S rRNA homology analysis of Lactobacillus isolated from Dali Chinese

作　　者：崔照琼[1] 郤翠仙[2] 周玉照 柴俊[2] 刘旭川[2] 张以芳[2] 

机构地区：[1]昆明医科大学海源学院细胞生物学暨医学遗传学教研室,云南昆明650106 [2]云南农业大学动物科技学院,云南昆明650201

出　　处：《中国微生态学杂志》2015年第4期394-399,共6页Chinese Journal of Microecology

基　　金：云南省重点新产品开发计划(2014BB004);云南省院士专家工作站基金(2014IC034);云南省应用基础研究重点项目(2011FA014);国家自然科学基金(31260598)

摘　　要：目的乳扇是云南大理白族的一种传统乳制品,明确大理乳扇制品中乳杆菌的多样性及优势种群分布,为科学利用奠定基础。方法采用表型鉴定及16S r RNA鉴定方法,对10个家庭作坊的大理乳扇制品中的乳杆菌进行了分离鉴定。结果共分离到50株乳杆菌,通过表型鉴定为8个种,包括植物乳杆菌10株、德氏乳杆菌7株、发酵乳杆菌6株、干酪乳杆菌6株、棒状乳杆菌4株、鼠乳杆菌2株、弯曲乳杆菌3株和食果糖乳杆菌2株;06422和06430两株表型鉴定未能定种,进一步通过16S r RNA鉴定为植物乳杆菌和马酒乳杆菌,06422株与植物乳杆菌L.arizonensin、L.pentosus和L.plantarum P158的同源性分别是100%、100%和99.9%,与乳杆菌属其它种的同源性为83.4%(L.gallinarum)至93.5%(L.brevis),06430株与L.kefiranofaciens.subsp.Kefirgranum的16S r RNA同源性是99.9%,与乳杆菌属其它种的同源性为83.7%(L.plantarum P158)至96.3%(L.acidophilus)。结论大理乳扇制品中有9种乳杆菌,其优势种群为植物乳杆菌、德氏乳杆菌、发酵乳杆菌和干酪乳杆菌等四种。Objective Dairy fan is a kind of delicate traditional food of Bai people in Dali Yunnan. The study maked it clear that diversity and dominant species distribution of lxtctobacillus in Dairy fan, and established a foun- dation for scientific use. Methods In the study, we used 16S rRNA and phenotypic identification methods. Results Fifty Lactobacillus strains were isolated from 10 samples of traditionally home-made Dairy fan. Phenotypie identification results were eight species, most of them were identified as Lb. plantarum （ 10 strains ） , Lb. del- brueckii（ 7 strains）, Lb. fermentrum （ 6 strains）, Lb. casei（ 6 strains）, Lb. corymiformis （4 strains）, Lb. murinus （2 strains）, Lb. curvatus （3 strains） , Lb. fructivora （2 strains）. But 06422 and 06430 were not identified as spe- cies. The result of 16S rRNA analysis indicated that 06422 was primarily identified as a member of L. plantarum and 06430 as L. kefiranofaciens, subsp, kefirgranum. T, and 06422 strain shared 100% , 100% and 99. 9% ho-mology with L. arizonensin, L. pentosus and L plantarum P158 respectively, and the homology with others Lactoba- cillus were 83.4% （ L. gallinarum） to 93.5% （ L. brevis） , 06430 strain shared 99.9% with L. kefiranofaciens, sub- sp. Kefirgranum, and the homology with others Lactobacillus were 83.7% （L. plantarum P158 ） to 96. 3% （L. aci- dophilus）. Conclusion Dairy fan products includes nine Lactobacillus species, and the dominant populations are Lactobacillus plantarum, Lactobacillus delbrueckii, Lactobacillus fermentum and Lactobacillus casei.

关 键 词：乳酸杆菌 分离鉴定 16S RRNA 

分 类 号：R378.992[医药卫生—病原生物学]