Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms  

作　　者：Zhi-yuan Guo Xun Sun Xiao-long Xu Qing Zhao Jiang Peng Yu Wang 

机构地区：[1]Institute of Orthopedics,Chinese PLA General Hospital [2]The Neural Regeneration Co-innovation Center of Jiangsu Province

出　　处：《Neural Regeneration Research》2015年第4期651-658,共8页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.31100696,31170946;a grant from the National High Technology Research and Development Program of China(863 Program),No.2012AA020502;a grant from the National Program on Key Basic Research Project of China(973 Program),No.2014CB542201;a grant from Beijing Metropolis Beijing Nova Program,No.2011115

摘　　要：Human umbilical cord-derived mesenchymal stem cells （hUCMSCs） represent a promising young-state stem cell source for cell-based therapy. hUCMSC transplantation into the transected sciatic nerve promotes axonal regeneration and functional recovery. To further clarify the para-crine effects of hUCMSCs on nerve regeneration, we performed human cytokine antibody array analysis, which revealed that hUCMSCs express 14 important neurotrophic factors. Enzyme-linked immunosorbent assay and immunohistochemistry showed that brain-derived neurotrophic factor, glial-derived neurotrophic factor, hepatocyte growth factor, neurotrophin-3, basic fibroblast growth factor, type I collagen, fibronectin and laminin were highly expressed. Treatment with hUCMSC-conditioned medium enhanced Schwann cell viability and proliferation, increased nerve growth factor and brain-derived neurotrophic factor expression in Schwann cells, and enhanced neurite growth from dorsal root ganglion explants. These ifndings suggest that paracrine action may be a key mechanism underlying the effects of hUCMSCs in peripheral nerve repair.Human umbilical cord-derived mesenchymal stem cells （hUCMSCs） represent a promising young-state stem cell source for cell-based therapy. hUCMSC transplantation into the transected sciatic nerve promotes axonal regeneration and functional recovery. To further clarify the para-crine effects of hUCMSCs on nerve regeneration, we performed human cytokine antibody array analysis, which revealed that hUCMSCs express 14 important neurotrophic factors. Enzyme-linked immunosorbent assay and immunohistochemistry showed that brain-derived neurotrophic factor, glial-derived neurotrophic factor, hepatocyte growth factor, neurotrophin-3, basic fibroblast growth factor, type I collagen, fibronectin and laminin were highly expressed. Treatment with hUCMSC-conditioned medium enhanced Schwann cell viability and proliferation, increased nerve growth factor and brain-derived neurotrophic factor expression in Schwann cells, and enhanced neurite growth from dorsal root ganglion explants. These ifndings suggest that paracrine action may be a key mechanism underlying the effects of hUCMSCs in peripheral nerve repair.

关 键 词：nerve regeneration human umbilical cord-derived mesenchymal stem cells conditioned medium Schwann cells dorsal root ganglion AXONS peripheral nerve regeneration neurotrophic factors neural regeneration 

分 类 号：R741[医药卫生—神经病学与精神病学]