核酸适配体修饰的铁纳米粒对EGFR阳性肿瘤细胞的靶向热疗效应  

Enhancement of thermal damage to EGFR-positive U251 cancer cells by aptamer modified magnetic nanoparticle

作　　者：于连元[1] 胡燕[1] 段金虹[1] 许海燕[2] 杨先达[1] 

机构地区：[1]中国医学科学院基础医学研究所病理学与病理生理学系,北京100005 [2]中国医学科学院基础医学研究所生物医学工程学系,北京100005

出　　处：《基础医学与临床》2015年第5期637-641,共5页Basic and Clinical Medicine

基　　金：科技部重大科学计划(2011CB933504)

摘　　要：目的构建一种核酸适配体修饰的铁纳米粒子(Apt-NP),并研究其对表皮生长因子受体(EGFR)阳性的人胶质瘤细胞的靶向热疗效应。方法用流式细胞术检测核酸适配体的特异性,用生物素与链霉素的结合反应的原理构建Apt-NP,用DNA探针杂交检测核酸适配体是否结合在纳米粒子表面,用动态光散射粒径仪观察Apt-NP的尺寸分布,普鲁士蓝染色检测Apt-NP的靶向结合能力,通过检测磁场条件下细胞释放的LDH来评估Apt-NP的靶向热疗效应。结果 Apt-NP的平均直径为574 nm,较强结合EGFR阳性U251肿瘤细胞。此外,在磁场条件下,Apt-NP可以显著提升对EGFR阳性U251肿瘤细胞的热疗杀伤效应(P<0.05)。结论 Apt-NP可以显著提升针对EGFR阳性U251肿瘤细胞的热疗杀伤作用,在肿瘤靶向热疗方面具有潜在的应用价值。Objective To develop a novel EGFR aptamer-modified magnetic nanoparticle（ Apt-NP） for enhancing the targeted thermal damage to EGFR-positive U251 cancer cells. Methods EGFR aptamer was implanted onto nanoparticles via biotin-streptavidin reaction. Apt-NP was characterized by dynamic light scattering（ DLS） assay.The binding of aptamer to target cell was evaluated by flow cytometry. The attachment of Apt-NP to target cell was evaluated by Prussian blue staining. Thermal damage under alternative magnetic field was measured by detecting the release of LDH. Results The average size of Apt-NP was 574 nm. Apt-NP could bind with the EGFR-positive U251 tumor cells but not the MDA-MB-231 cell. Importantly,Apt-NP significantly enhanced the thermal damage to EGFR-positive U251 tumor cells but not that to MDA-MB-231 cells. Conclusions Aptamer-modified nanoparticles may have potential utility in tumor targeted thermal therapies

关 键 词：核酸适配体 热疗 EGFR 人胶质细胞瘤 

分 类 号：R730.5[医药卫生—肿瘤]