乙型肝炎病毒前C区基因变异与肝细胞癌相关性的研究  

Correlation between hepatitis B virus precore gene mutation and hepatocellular carcinoma

作　　者：胡艳芳[1] 王淼[1] 史为涛[1] 王勤英[1] 

机构地区：[1]山西医科大学第一医院感染病科,太原030001

出　　处：《中华实验和临床病毒学杂志》2015年第2期103-105,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：山西省教育厅项目编号（20121006）

摘　　要：目的 探讨乙型肝炎病毒（HBV）前C区基因变异与肝细胞癌（HCC）发生的关系.方法 收集在山西医科大学第一医院感染病科门诊及住院的患者血清及病例资料共99例,其中慢乙肝55例,肝癌44例.采用半巢式聚合酶连反应及直接测序法检测前C区基因变异的情况.分别检测两组患者的乙肝病毒标志物、HBV DNA,采用病例对照研究方法对患者的年龄、性别、HBeAg、HBVDNA基线水平和HBV前C区基因变异位点进行分析.结果 （1）肝癌患者的HBeAg阴性率均高于慢乙肝组,差异有统计学意义（P＜0.05）.（2）肝癌组患者HBV DNA载量低于慢乙肝组,差异有统计学意义（P＜0.05）.（3）共发现前C区有G1896A、G1899A两个热点突变位点,其中G1896A位点在肝癌组的突变率显著高于慢乙肝组,差异有统计学意义（P＜0.05）,而G1899A在两组之间差别无统计学意义.（4）另在前C区发现有插入及缺失突变,均相对点突变发生率较低.结论 HBV前C基因区G1896A位点突变与HCC发生密切相关.Objective The aim of the study was to investigate the mutation of precore （PreC） region of hepatitis B virus （HBV） gene and its association with hepatocellular carcinoma.Methods A total of 99 HBV patients were divided into two groups：chronic hepatitis B （CHB,55） and hepatocellular carcinoma （HCC,44）.The precore gene fragment was amplified by nested PCR and analyzed by direct DNA sequencing.To analyze the relationships between HCC occurrence and age,sex,baseline HBV DNA,HBeAg status,precore region gene mutation was analyzed in two groups by using the method of case-control.Results There was significant difference between the patients with hepatocellular carcinoma and chronic HBV infection in the baseline levels of HBV DNA.In HBV precore promote region,the common point mutations were G1896A and G1899A.Compared with patients with chronic hepatitis B,point mutation G1896A,patients with liver cancer had a higher incidence of point mutation G1896A.The negative rate of HBeAg in patients with mutation with G1896A was much higher in CHB group than HCC group,the difference was significant （P 〈 0.05）.There was no significant difference between hepatocellular carcinoma and chronic HBV infection in the mutation rate of G1899A （P 〉 0.05）.The rate of insertion or deletion mutations in HBV DNA Precore gene were lower than point mutation.Conclusion HBV PC 1896 mutation was correlated with the occurrence of hepatocellular carcinoma.

关 键 词：癌 肝细胞 肝炎病毒 乙型 基因 突变 

分 类 号：R735.7[医药卫生—肿瘤]