双氢杨梅树皮素对人视网膜母细胞瘤HXO-RB44细胞色素C氧化酶表达的影响  

Influence of ampelopsin on expression of cytochrome C oxidase of human retinoblastoma HXO-RB44 cells

作　　者：蒋林志[1] 梁皓[1] 严宇清[1] 陈迎迎[1] 唐琪[2] 彭格员 

机构地区：[1]广西医科大学第一附属医院眼科,南宁市530021 [2]广西医科大学,2009级研究生南宁市530021

出　　处：《广西医学》2015年第3期356-358,367,共4页Guangxi Medical Journal

基　　金：广西自然科学基金(2011GXNSFA018243);广西中医药管理局项目(GZZC1052)

摘　　要：目的探讨双氢杨梅树皮素(APS)对体外培养的视网膜母细胞瘤(RB)细胞中细胞色素C氧化酶蛋白IV(COX-IV)表达的影响。方法取对数生长期的人RB细胞株(HXO-RB44)进行体外培养,根据APS药物浓度设3个组:4.40μg/ml(A组)、14.84μg/ml(B组)、33.33μg/ml(C组),并设空白对照组及顺铂(2.00μg/ml)阳性对照组。采用蛋白质印迹分析(Western Blot法)测定各组COX-IV的表达量。结果不同药物浓度组APS干预体外培养的RB细胞24 h后,COX-IV的表达量均明显降低;各APS药物组与空白对照组、阳性对照组比较,差异均有统计学意义(P<0.05),其中A组降低COX-IV活性表达最为明显,相对灰度值为(0.112±0.032);各组间并不呈现随药物浓度增加,COX-IV活性表达降低越明显的特征。结论 APS能降低COX-IV的表达量,COX-IV的表达可能存在启动阈值,其表达量并不随APS药物浓度升高而降低。Objective To explore the influence of ampelopsin（APS） on the expression of cytochrome C oxidase IV（COX-IV） of human retinoblastoma（RB） eell cultured in vitro. Methods Human retinoblastoma RB cell lines （HXO-RB44） cultured in vitro were divided into three groups according the APS concentration,group A （4.40μg/ml） ,group B （14.84 μg/ml） and group C（33.33 μg/ml）. The blank control group and positive control group （cisplatin, 2.00 μg/ml） were enrolled in the study. The COX-IV expression was determined in each group by Western Blot. Results The COX-IV expression decreased significantly in each APS group after RB cells cultured in vitro had been treated for 24 hours, which showed a significant difference among each APS group, blank control group and positive control group（ P 〈 0.05 ）. The decrease of COX-IV activity expression was the most significant in group A,and the relative grey value was （0. 112 ±0. 032）. No group presented a characteristic of higher drug concentration and lower COX-IV activity expression. Conclusion APS can reduce the expression of COX-IV. The COX-IV expression may own the threshold, and its expression doesn＇t decrease with the increasing APS concentration.

关 键 词：视网膜母细胞瘤 双氢杨梅树皮素 细胞色素C氧化酶 藤茶 

分 类 号：R774[医药卫生—眼科]