富硒和γ-氨基丁酸萌芽糙薏米培养液组分的工艺优化  

Process Optimization on Enriched Selenium andγ-amino-butyric Acid in the Germination Cultures for the Unpolished Coix Seed

作　　者：党娟[1] 秦礼康[1] 杨先龙 金毅[1] 李谦[1] 张素云[1] 

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州鑫龙食品开发有限公司,贵州安顺561000

出　　处：《贵州农业科学》2015年第4期182-186,共5页Guizhou Agricultural Sciences

基　　金：贵州省农业攻关项目(黔科合农G字20124001);贵州省科技重大专项"薏米精深加工产业化关键技术提升与应用"(黔科合字20146023);贵州省科技重大专项"科特派员特色苦荞创业链建设与示范"(黔科合重大专项字20136010-5);省县市三方合作项目"薏米加工副产物循环增值利用研究与产业化示范"[安西科合gzassxx1ch20123006(1)];安顺市科技局项目[安市科合2012(5)]

摘　　要：为提高糙薏米中γ-氨基丁酸的富集量,采用响应面试验法对糙薏米的培养液组分进行工艺优化。结果表明:培养液中Na2SeO3浓度以10mg/L为宜,其发芽率最高,达(90.33±1.73)%;在Na2SeO3浓度为10mg/L的培养液中辅以11mmol/L CaCl2、87.7μmol/L GA3和31.5mmol/L MSG进行萌芽处理时,萌芽糙薏米中总硒、有机硒和GABA含量分别达0.42mg/100g、0.37mg/100g和116.69mg/100g,是一种具高值化产品开发潜力的保健食品基料。To improve the enrichment amount of γ-amino-butyric acid from unpolished coix seed, Response surface methodology （RSM）was used to optimize the culture components of unpolished coix seed.The results showed that the concentration of Na2 SeO3 in the culture medium to 10 mg/L is appropriate,the highest germination rates were up to （90.33±1.73）%.And combined with the RSM optimization results Na2 SeO3 10 mg/L,CaCl2 11 mmol/L,GA3 87.7μmol/L and MSG 31.5 mmol/L,the total selenium,organic selenium and GABA in the germinated unpolished coix seed were up to 0.42 mg/100g,0.37 mg/100g and 116.69 mg/100g,respectively.It’s a good material with high-value potential for health food development.

关 键 词：糙薏米 Γ-氨基丁酸 硒 萌芽 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]