The prolactin family hormones regulate vascular tone through NO and prostacyclin production in isolated rat aortic rings  

作　　者：Carmen GONZALEZ Hector ROSAS-HERNANDEZ Brenda JURADO-MANZANO Manuel Alejandro RAMIREZ-LEE Samuel SALAZAR-GARClA Pedro Pablo MARTINEZ-CUEVAS Aida Jimena VELARDE-SALCEDO Humberto MORALES- LOREDO Ricardo ESPINOSA-TANGUMA Syed F ALl Rafael RUBIO 

机构地区：[1]Universidad Autonoma de San Luis Potosi, Facultad de Ciencias Quimicas, San Luis Potosi 78210, Mexico [2]Universidad Autonoma de San Luis Potosi, Facultad de Medicina, San Luis Potosf 78210, Mexico [3]Neurochemistry Laboratory, Division of Neurotoxicology, National Center for Toxicological Research/FDA, Jefferson, AR, USA

出　　处：《Acta Pharmacologica Sinica》2015年第5期572-586,共15页中国药理学报（英文版）

摘　　要：Aim： Prolactin family hormones include growth hormone, placental lactogen and prolactin, which are able to regulate angiogenesis via NO and prostaglandins. However, their effects on vascular tone are not fully understood. The aim of this study was to evaluate the effects of prolactin family hormones on rat vascular tone in vitro. Methods： Aortic rings were prepared from adult male rats and precontracted with phenylephrine, then treated with the hormones and drugs. The tension was measured with isometric force displacement transducer connected to a polygraph. NO production and prostacyclin release in physiological solution was determined. Cultured rat aortic endothelial cells （RAECs） were treated with the hormones and drugs, and the phosphorylation of eNOS at serine 1177 was assessed using Western bolt analysis. Results： Administration of growth hormone or placental lactogen （0.01-100 nmol/L） induced endothelium-dependent vasodilation. Both the hormones significantly increased the phosphorylation of eNOS in RAECs and NO level in physiological solution. Preincubation with L-NAME blocked growth hormone- or placental lactogen-induced vasodilation and NO production. Preincubation with an antibody against growth hormone receptors blocked growth hormone- and placental lactogen-induced vasodilation. Addition of a single dose of prolactin （0.01 nmol/L） induced sustained vessel relaxation, whereas multiple doses of prolactin induced a biphasic contraction- relaxation effect. The vascular effects of prolactin depended on endothelium. Prolactin significantly increased the level of prostacyclin 12 in physiological solution. Preincubation with indomethacin or an antibody against prolactin receptors blocked prolactin-induced vasodilation.Conclusion： The prolactin family hormones regulate rat vascular tone, selectively promoting either relaxation or contraction of vascular smooth muscle via activation of either growth hormone receptors or prolactin receptors within the endothelium.Aim： Prolactin family hormones include growth hormone, placental lactogen and prolactin, which are able to regulate angiogenesis via NO and prostaglandins. However, their effects on vascular tone are not fully understood. The aim of this study was to evaluate the effects of prolactin family hormones on rat vascular tone in vitro. Methods： Aortic rings were prepared from adult male rats and precontracted with phenylephrine, then treated with the hormones and drugs. The tension was measured with isometric force displacement transducer connected to a polygraph. NO production and prostacyclin release in physiological solution was determined. Cultured rat aortic endothelial cells （RAECs） were treated with the hormones and drugs, and the phosphorylation of eNOS at serine 1177 was assessed using Western bolt analysis. Results： Administration of growth hormone or placental lactogen （0.01-100 nmol/L） induced endothelium-dependent vasodilation. Both the hormones significantly increased the phosphorylation of eNOS in RAECs and NO level in physiological solution. Preincubation with L-NAME blocked growth hormone- or placental lactogen-induced vasodilation and NO production. Preincubation with an antibody against growth hormone receptors blocked growth hormone- and placental lactogen-induced vasodilation. Addition of a single dose of prolactin （0.01 nmol/L） induced sustained vessel relaxation, whereas multiple doses of prolactin induced a biphasic contraction- relaxation effect. The vascular effects of prolactin depended on endothelium. Prolactin significantly increased the level of prostacyclin 12 in physiological solution. Preincubation with indomethacin or an antibody against prolactin receptors blocked prolactin-induced vasodilation.Conclusion： The prolactin family hormones regulate rat vascular tone, selectively promoting either relaxation or contraction of vascular smooth muscle via activation of either growth hormone receptors or prolactin receptors within the endothelium.

关 键 词：ENDOTHELIUM growth hormones placental lactogen PROLACTIN eNOS NO PROSTAGLANDINS aortic rings aortic endothelial cells 

分 类 号：Q575.11[生物学—生物化学] S828[农业科学—畜牧学]