ErbB3和Flotillin-2在骨肉瘤中的表达及临床病理意义  

EXPRESSION OF ErbB3 AND Flotillin-2 AND CLINICAL PATHOLOGICAL SIGNIFICANCE IN OSTEOSARCOMA

作　　者：刘雨微[1] 成亮[1] 朱剑熹[1] 杨程[2] 肖凯[1] 罗伟[1] 高曙光[1] 李康华[1] 

机构地区：[1]中南大学湘雅医院骨科,长沙410008 [2]中南大学湘雅医学院附属海口医院骨科

出　　处：《中国修复重建外科杂志》2015年第5期590-594,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家高技术研究发展计划(863)资助项目(2011AA030101)~~

摘　　要：目的探讨ErbB3和Flotillin-2在骨肉瘤中的表达及其相关性,并分析其临床意义。方法取2009年9月-2014年3月收治的38例骨肉瘤患者和13例骨软骨瘤患者样本组织,行免疫组织化学染色。镜下观察ErbB3和Flotillin-2的表达情况,并统计分析二者在骨肉瘤和骨软骨瘤中阳性表达差异性、二者相关性及其与临床特征的关系。结果镜下观察示,ErbB3、Flotillin-2蛋白均在骨肉瘤组织细胞质中高表达,其阳性率显著高于骨软骨瘤组织中(P<0.05)。ErbB3和Flotillin-2在骨肉瘤中的表达具有较好一致性(Kappa值=0.434,P=0.000)。ErbB3在骨肉瘤中表达与患者性别、年龄、肿瘤部位、肿瘤大小不相关(P>0.05),与美国国立综合癌症网络(NCCN)指南骨肉瘤临床分期及肺转移显著相关(P<0.05);而Flotillin-2在骨肉瘤中的表达与以上因素均无相关(P>0.05)。影响骨肉瘤患者总生存期的因素包括骨肉瘤临床分期、肺转移、ErbB3表达阳性及肿瘤大小(P<0.05),独立影响因素是肺转移和ErbB3表达阳性(P<0.05)。结论 ErbB3和Flotillin-2在骨肉瘤中过表达,且具有一致性。此外,ErbB3的表达与骨肉瘤临床分期和肺转移有关,肺转移和ErbB3过表达提示预后不佳。Objective To investigate the expression of ErbB3 and Flotillin-2 in osteosarcoma biopsies and possible clinical pathology significance.Methods The tissue biopsies were harvested from 38 osteosarcoma patients and 13 osteochondroma patients between September 2009 and March 2014 for immunohistochemical staining.The ErbB3 and Flotillin-2 expressions were observed in osteosarcoma and osteochondroma biopsies,and the correlation and the relationship to the clinical and pathological features were analyzed.Results The expression levels of ErbB3 and Flotillin-2 in osteosarcoma were significantly higher than those in osteochondroma（P〈0.05）.The high expressions of ErbB3 and Flotillin-2 had good consistency in osteosarcoma（Kappa=0.434,P=0.000）.The high expression of ErbB3 was positively correlated to the clinical stages and lung metastasis（P〈0.05）,but it was not associated with gender,age,tumor location,and size（P〉0.05）.The high expression of Flotillin-2 had no correlation with clinical and pathological features（P〉0.05）.The influence factors of patients’ overall survival included clinical stages,lung metastasis,high expression of ErbB3,and tumor size（P〈0.05）.Only lung metastasis and high expression of ErbB3 were independent factor affecting overall survival（P〈0.05）.Conclusion The ErbB3 and Flotillin-2 express highly in osteosarcoma and the high expression has good consisitency.Besides,the high expression of ErbB3 is associated with the clinical stages and lung metastasis,indicating a poor prognosis for osteosarcoma.

关 键 词：ERBB3 Flotillin-2 骨肉瘤 免疫组织化学 

分 类 号：R738.1[医药卫生—肿瘤]