山苍子油成分分析及其对日化产品腐败菌的抑制效果研究  

Analysis of components in Litsea cubeba oil and their effect for inhibition of putrilage bacteria in daily chemical products

作　　者：梁青[1] 李文茹[1] 施庆珊[1] 章卫民[1] 

机构地区：[1]广东省微生物研究所广东省微生物分析检测中心华南应用微生物国家重点实验室广东省菌种保藏与应用重点实验室,广东广州510070

出　　处：《日用化学工业》2015年第5期260-264,268,共6页China Surfactant Detergent & Cosmetics

基　　金：国家自然科学基金资助项目(21401028);揭阳市产学研结合项目(201429)

摘　　要：通过水蒸气蒸馏法提取山苍子油,采用气相色谱-质谱联用仪(GC-MS)分析其化学成分,并用琼脂平板法测定最低抑制浓度(MIC)以研究山苍子油对3株从日化产品中分离的腐败细菌的抑菌效果,同时根据16S rRNA序列的系统进化树对3株菌进行菌株鉴定。结果表明:山苍子油主要成分是D-柠檬烯(w=26.51%)、柠檬醛(w=11.94%)和马鞭烯醇(w=11.84%);3株菌均为革兰氏阳性菌,分别命名为解淀粉芽孢杆菌GLM 50、萎缩芽孢杆菌GLM 56和溶血葡萄球菌GLM 57;琼脂平板法表明山苍子油对3株菌的最低抑制浓度分别为0.045,0.09和0.18 g/L。Litsea cubeba oil was obtained by steam distillation of fruit of Litsea cubeba（ Lour.） Pers. and their components were identified by gas chromatography / mass spectrometry（ GC- MS）. Examination of inhibition effect of Litsea cubeba oil against three strains of bacterium isolated from daily chemical products was based on data of minimum inhibitory concentrations（ MICs） determined by agar plane dish test. Meanwhile,the three strains of bacterium were appraised in accordance with 16 S rRNA sequence analyses to determine their phylogenetic positions. Results showed that the main constituents of Litsea cubeba oil are D- limonene（ w =26. 51%）,citral（ w = 11. 94%） and verbenol（ w = 11. 84%）. All the three strains belong to gram- positive bacteria and are classified as Bacillus amyloliquefaciens subsp. plantarum GLM 50,Bacillus atrophaeus GLM 56 and Staphyococcus haemolyticus GLM 57 respectively. The MICs of Litsea cubeba oil against GLM 50,GLM 56 and GLM 57 are 0. 045,0. 09 and 0. 18 g / L respectively.

关 键 词：山苍子油 气质联用 菌株鉴定 腐败菌抑制 

分 类 号：TQ047.6[化学工程]