一株产朊假丝酵母10L罐发酵工艺优化  

10 L Fermentation Medium for the Culture of Candida utilis

作　　者：齐宏亮[1,2] 崔卫东[1] 包慧芳[1] 詹发强[1] 王炜[3] 

机构地区：[1]新疆农业科学院微生物应用研究所,乌鲁木齐830091 [2]赤峄制药股份有限公司,内蒙古赤峄024005 [3]中国科学院新疆分院,乌鲁木齐830011

出　　处：《新疆农业科学》2015年第4期718-723,共6页Xinjiang Agricultural Sciences

基　　金：自治区高技术项目(201111112);自治区国际合作项目(20116005);国家国际科技合作计划(2010DFA92720-11-4);科技支疆项目(201091227);乌鲁木齐市种子基金“饲用微生物添加剂的研制”(K111410004)

摘　　要：【目的】优化产朊假丝酵母10 L罐发酵条件,提高产朊假丝酵母发酵产率。【方法】在10 L发酵罐中研究溶氧量和p H控制方式对产朊假丝酵母分批发酵的影响。【结果】当装液量为7 L且通气量控制在8 L/min、搅拌转速达到300 r/min即可满足产朊假丝酵母生长对溶氧的需求。通过流加碱的方式控制发酵p H环境,结果显示,流加氨水控制p H在5.5时最佳。【结论】通过优化发酵溶氧量和p H,产朊假丝酵母干重产量提高了54%,优化成效明显。【Objective】To optimize the high-density culture growth methods of Candida utilis,in order to improve the production of it. 【Method】This experiment is designed to analyze the effects of different dissolved oxygen and p H on the batch fermentation of Candida utilis.【Result】The results showed that when the liquid volume was 7 L and a 8 L/min air flow rate,and the agitation rate was more than 300 r/min,the cell growth would be enhanced significantly. The optimal control p H was 5. 5. 【Conclusion】By optimizing the fermentation amount of dissolved oxygen and p H,Candida utilis was improved by 54%,achieving remarkable result and could be generalized to promote production.

关 键 词：产朊假丝酵母 高密度发酵 发酵工艺 益生菌 

分 类 号：S188[农业科学—农业基础科学]