杏鲍菇多糖超声辅助酶法提取条件优化  

Optimization on ultrasonic and enzymatic extraction of polysaccharides from Pleurotus eryngii

作　　者：徐珂[1] 柯乐芹[1] 肖建中[1] 张东旭[1] 

机构地区：[1]丽水学院,浙江丽水323000

出　　处：《浙江农业学报》2015年第4期647-651,共5页Acta Agriculturae Zhejiangensis

基　　金：浙江省十二五重大专项(2012C12012-1);浙江省丽水市科技计划项目(2011NZH0209)

摘　　要：以杏鲍菇下脚料为原料,利用超声波或纤维素酶进行处理,以无水乙醇为提取剂,提取杏鲍菇多糖。以杏鲍菇多糖得率为优化参数,通过设计的响应面试验和正交试验对提取工艺进行优化,同时将超声提取和酶法提取进行有效组合,试验结果表明,超声提取的最佳条件为:水料比30∶1,p H 6.0,超声波功率100 W,提取时间20 min,多糖得率为22.17%;酶法提取的最佳条件为:水料比25∶1,加酶量0.80%,酶解温度60℃,酶解时间120 min,p H 5.5,多糖得率为26.48%;当两种提取方法组合时,以先超声后酶法提取的多糖得率最高,为30.46%,是传统水提法的1.92倍。Polysaccharide was extracted from Pleurotus eryngii wastes processed by ultrasonic or cellulase, with ethanol as extraction agent. Response surface experiments and orthogonal design were used to optimize the extraction process. Ultrasonic extraction and enzymatic extraction, both with the optimal parameters, were effectively combined to get the highest Pleurotus eryngii polysaccharide yield. The results showed that, the optimal ultrasonic extraction conditions were： water material ratio 30： 1, pH 6. 0, ultrasonic power 100 W, extraction time 20 min, the yield of polysaccharide was 22. 17%. The optimal conditions of enzymatic extraction were： water material ratio 25： 1, the amount of enzyme 0. 80% , enzymatic hydrolysis temperature 60℃, enzymatic hydrolysis time 120 min, pH 5.5, the yield of polysaccharide was 26. 48%. The combinations of the two methods gave the highest yield of 30. 46%, which was 1.92 times higher than that by the traditional water extraction methhod.

关 键 词：杏鲍菇 多糖 超声波 纤维素酶 

分 类 号：S146.1[农业科学—肥料学] TQ914.3[农业科学—农业基础科学]