高效液相色谱法测定调制乳中低聚果糖的含量  

High Performance Liquid Chromatography Determination of Fructooligosaccharides in Modifi ed Milk

作　　者：万鹏 赵贞 刘春霞 岳虹 李翠枝 邵建波 

机构地区：[1]内蒙古伊利实业集团股份有限公司,内蒙古呼和浩特010110

出　　处：《乳业科学与技术》2015年第3期23-25,共3页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金："十二五"国家科技部支撑计划项目(2012BAK17B00)

摘　　要：建立调制乳中低聚果糖高效液相色谱的测定方法。采用水作为提取剂,乙腈沉淀蛋白,氨基色谱柱分离,流动相为70%乙腈溶液,示差检测器检测。每100 g调制乳中低聚果糖添加量为20-500 mg时,回收率为80.2%-107.1%,定量检出限为20 mg/100 g。该方法操作简便、检测准确,可用于调制乳中低聚果糖的检测。A method for the determination of fructooligosaccharides in modified milk by high performance liquid chromatography （HPLC） was developed. Fructooligosaccharides were extracted with water. The analysis was performed on a NH： column using 70% acetonitrile in water as the mobile phase. The recoveries of fructooligosaccharides at four spiked levels between 20 and 500 mg/100 g ranged from 80.2% to 107.1%. The limit of quantification was 20 mg/100 g. This method is accurate, simple and suitable for the analysis of fructooligosaccharides in modified milk.

关 键 词：高效液相色谱 低聚果糖 调制乳 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]