复叶耳蕨渣总黄酮转化提取与DPPH自由基清除活性的研究  

Extraction of total flavonoids in Arachniodes exilis dregs assisted by soil fungi

作　　者：张义平[1] 胡月萍[1] 邬亚华[1] 孙维君[1] 周许峰[1] 曹俊[1] 王丽丽[1] 殷嫦嫦[1] 

机构地区：[1]九江学院基础医学院,江西九江332000

出　　处：《食品工业科技》2015年第11期100-105,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金(81360364)

摘　　要：研究复叶耳蕨渣的土壤真菌发酵转化提取其总黄酮和清除DPPH自由基活性。复叶耳蕨渣直筛发酵用优势菌株;通过单因素和正交实验L9(34)优化总黄酮提取,DPPH法评价转化前后总黄酮抗氧化活性。研究得到优势菌株F2发酵复叶耳蕨渣后黄酮得率为(0.893%±0.035%),较未发酵时总黄酮得率提高了27.0%,差异显著。F2发酵复叶耳蕨渣在最佳提取条件(料液比1∶30(g/m L),乙醇浓度70%,时间40min)下总黄酮产量较发酵前提高了30.6%。复叶耳蕨渣F2发酵前后总黄酮均表现出优于VE的清除DDPH自由基能力,但发酵前后总黄酮清除DPPH自由基能力无显著性差异。土壤真菌F2固体发酵增加了复叶耳蕨渣总黄酮的提取,复叶耳蕨渣黄酮提取物表现出比VE更强的清除DPPH自由基能力,作为一种天然抗氧剂具有良好的应用前景。The soil fungi-assisted extraction of total fiavonoids from Arachniodes dregs and its DPPH scavenging capacity was studied in the paper.Soil fungi were separated and puritied by the gradient dilution method.The Arachniodes dregs were fermented by them, and single factor test and orthogonal test L9 (3^4 ) were employed to optimize the best condition of extracting their total flavonoids by ultrasonic assistant.The antioxidant activities of total flavonoids were studied by DPPH scavenging test.The yield of the flavonoids was 0.893% ±0.035% by the method.Comparing with ultrasonic extraction,the extraction ratio of total flavonoids in Arachniodes dregs fermented by F2 were 27.0% higher than without fermentation. Under optimized extraction conditions of flavonoids with orthogonal test,which were solid to liquid ratio 1:30(g/mL) ,ethanol concentration 70% (v/v)and ultrasonic extract 40min,the yield of the fiavonoids was 30.6% higher than without fermentation.The total flavonoids extracted from Arachniodes dregs before and after fermentation possessed stronger scavenging effect on DPPH thanVE.The results showed that compared to ethanol extracting method, soil fungi fermentation could promote the yield of flavonoids significantly,total fiavonoids in Arachniodes dregs fermented by F2 are effective as natural antioxidants, which provided the basis of recycling the resources of Arachniodes dregs for future.

关 键 词：复叶耳蕨 土壤真菌 固体发酵 DPPH自由基清除 

分 类 号：TS284.2[轻工技术与工程—食品科学与工程]