菊芋秸秆的稀酸水解及乙醇发酵  

Ethanol production using stalk of Jerusalem artichoke by Kluyveromyces marxianus

作　　者：相瑞娟 尹思静[1] 侯胜博 袁文杰[1] 

机构地区：[1]大连理工大学生命科学与技术学院,辽宁大连116023

出　　处：《现代化工》2015年第5期81-84,共4页Modern Chemical Industry

基　　金：国家自然科学基金(21106016);中央高校业务费(DUT14LK33)

摘　　要：菊芋作为一种非粮作物,块茎和秸秆均可以被微生物发酵生成乙醇。采用稀酸法对菊芋秸秆进行预处理,通过单因素实验,考察了预处理温度、预处理时间、稀酸浓度、料液比4个因素,得到的优化结果:料液比为1∶8,酸解温度为121℃,酸质量分数为1.5%,酸解时间为1 h。此条件下水解菊芋秸秆,还原糖得率高达53.7%;预处理后的水解液在添加纤维素酶和木聚糖酶后,考察Kluyveromyces marxianus 1727的乙醇发酵能力,其同步糖化发酵与分步糖化发酵乙醇产量分别为25.91 g/L和25.63 g/L,生产效率分别是0.54 g/L/h和0.26 g/L/h。结果表明,稀酸水解的菊芋秸秆可用作底物生产燃料乙醇。As a kind of non-food crop, the tuber and stalk of Jerusalem artichoke can be used to produce ethanol by microorganism. The production of ethanol has been well investigated using Jerusalem artichoke tuber. In this study, Jerusalem artichoke stalks are pretreated by dilute acid. The effects of ratio of solid to liquid （ w/w）, concentration of acid, pretreatment temperature and reaction time on conversion rate of reducing sugar are studied. The optimum condition of pretreatment is achieved as follows：l： 8 of the ratio of solid to liquid, 1.5 % of the concentration of sulphuric acid, 121 ~C of reaction temperature and 60 minutes of reaction time. Under the optimum condition,the conversion rate of reducing sugar in the hydrolysate can achieve 53.7%. After adding cellulase and xylanase, the hydrolysis of Jerusalem artichoke stalks is used to produce ethanol by separate hydrolysis fermentation （SHF） and simultaneous saccharification and fermentation （SSF） with Kluyveromyces marxianus 1727. K. marxianus 1727 can use both the glucose and xylose in the stalk,and the concentrations of ethanol with SSF and SHF are 25.91 g/L and 25.63 g/L, respectively. Their ethanol productivity is 0. 54 g/L/h and 0. 26 g/L/h, respectively.

关 键 词：菊芋秸秆 酸水解 马克斯克鲁维酵母 同步糖化发酵 分布糖化发酵 

分 类 号：Q815[生物学—生物工程]