超临界CO_2萃取法提取棘托竹荪中的活性物质及其抑菌效果研究  

Supercritical CO_2 Extraction and Antibacterial Effects of Active Substances from Dictyophora echinovolvata

作　　者：蓝蔚青[1] 曹奕[1] 陈燕[1] 潘迎捷[1] 孙晓红[1] 

机构地区：[1]上海海洋大学食品学院上海水产品加工及贮藏工程技术研究中心,上海201306

出　　处：《天然产物研究与开发》2015年第5期832-836,858,共6页Natural Product Research and Development

基　　金：上海市教育委员会科研创新项目(11YZ159);上海市2015年科技兴农重点攻关项目(2015-4-12);上海海洋大学科技发展专项基金(A2-0209-15-200061)

摘　　要：本文研究了超临界CO2萃取法的不同提取条件对棘托竹荪中活性物质的抑菌效果影响。通过L16(43)正交实验,选取萃取压力、萃取温度与萃取时间为主要影响因素,采用超临界CO2萃取法,以活性物质对单增李斯特菌和副溶血性弧菌的最小抑菌浓度(MIC)与抑菌率作为提取效果指标,设计棘托竹荪抑菌活性物质的最佳提取条件。由不同提取条件对两种菌抑菌效果的影响程度与显著性分析得出:萃取时间>萃取温度>萃取压力。棘托竹荪活性物质提取的最佳条件为:萃取压力为20 MPa,萃取温度35℃,萃取时间120 min,所得活性物质对单增李斯特菌与副溶血性弧菌的MIC均为15.0 mg/m L。当抑菌活性物质浓度为MIC时,处理24 h后其对单增李斯特菌与副溶血性弧菌的最佳抑制率分别为85.7%与98.2%。In this paper,the antibacterial effects of active substances from Dictyophora echinovolvata prepared by supercritical CO2 extraction with different extraction conditions were studied. The orthogonal experiment L16（ 43） was designed for the optimization of extraction conditions of active substances. Extraction pressure（ EP）,extraction temperature（ ET1） and extraction time（ ET2） were used as the main factors in supercritical CO2 extraction. The antibacterial effects of active substances of D. echinovolvata against Listeria monocytogenes and Vibrio parahaemolyticus were measured by minimal inhibitory concentration（ MIC） and inhibitory rate. From the antibacterial effects of active substances and significance analysis for different extraction conditions,the results showed that： ET2 〉EP 〉ET1. The optimal extraction conditions were 20 MPa,35 ℃ and 120 min. The MICs of active substances against L. monocytogenes and V. parahaemolyticus were both 15. 0 mg / m L after 24 h incubation. When the concentrations of active substances were at MIC,the inhibitory rates against L. monocytogenes and V. parahaemolyticus were 85. 7% and 98. 2%,respectively.

关 键 词：超临界CO2萃取 棘托竹荪 最小抑菌浓度 抑菌活性 

分 类 号：Q93-33[生物学—微生物学]