婴幼儿配方乳粉中双歧杆菌的分子鉴定及标签相符性研究  

Study on molecular identification and labeling of Bifidobacterium in infant formula milk powder

作　　者：段文锋[1] 刘洋[1] 陈雯雯[2] 张清平[1] 俞漪[1] 曲勤凤[1] 赵渝[2] 

机构地区：[1]上海市质量监督检验技术研究院国家食品质量监督检验中心(上海),上海200233 [2]上海师范大学生命与环境科学学院,上海200234

出　　处：《中国乳品工业》2015年第5期16-20,共5页China Dairy Industry

基　　金：国家自然科学基金项目(31301486);上海市自然科学基金项目(13ZR1439600)

摘　　要：完成了对我国市场常见益生菌婴幼儿配方乳粉标签标识情况的调查;利用16Sr RNA技术建立了基于双歧杆菌基因组序列的分子鉴定方法,对市场常见的19例益生菌乳粉中分离得到的28株双歧杆菌进行菌株分子鉴定和标签相符性分析,为该类产品的安全监管提供技术参考和建议。Infant formula milk powder is a kind of product which gets most attention of comsumers. Probiotic formula milk powder for in- fants is gradually being loved by the people for its function of regulation bowel function. In this study, common brands ofprobiotic formula milk powder for infant have been collected and analysed with their labeling situation. Using standard strains, molecular identification technol- ogy is established based on 16S rRNA sequence of bifidobacterium genome. 28 strains of bifidobacterium separated from 19 probiotic milk powder are identificated and the accordance situation with label is analysed by apphcation of this molecular identification technology. This study provide technique and suggestion for safety supervision of the probiotic formula milk powder products.

关 键 词：16Sr RNA 双歧杆菌 分子鉴定 标签 

分 类 号：Q93-331[生物学—微生物学]