重组骆驼蓬脂转移蛋白对宫颈癌HeLa细胞化疗的增敏作用  

Sensitization of chemotherapy for human cervical carcinoma HeLa cells by recombinant Peganum harmala lipid transfer protein

作　　者：管珊[1] 王燕[1] 赵晶[1] 彭永玉[1] 吕慧[1] 孙素荣[1] 

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,新疆乌鲁木齐830046

出　　处：《生物技术》2015年第2期180-184,共5页Biotechnology

基　　金：新疆维吾尔自治区科技支疆项目("新疆骆驼蓬蛋白抗肿瘤作用的研究";No.20091129);新疆生物资源基因工程重点实验室开放基金项目("新疆骆驼蓬抗肿瘤蛋白活性的研究";No.XJDX0201-2010-06)资助

摘　　要：[目的]研究重组骆驼蓬脂转移蛋白(recombinant Peganum harmala lipid transfer protein,r Ph LTP)与顺铂(DDP)协同对宫颈癌He La细胞的抑制作用及可能的作用机制。[方法]四甲基偶氮咗蓝(MTT)法检测He La细胞生长的抑制率;Annexin-V/PI双染法检测细胞凋亡率,流式细胞术检测细胞内活性氧(ROS)的变化情况。[结果]r Ph LTP与DDP联合应用后与相应的DDP组相比,对细胞生长的抑制率显著增强(P<0.01),低浓度r Ph LTP联合2.5μg/m L顺铂对He La细胞的抑制率为(42.50±0.059)%,远高于高浓度顺铂(10μg/m L)对He La细胞的抑制率(36.88±0.134)%,细胞的凋亡率显著增加(P<0.01),细胞内ROS极明显升高(P<0.001)。[结论]r Ph LTP能够显著增强顺铂对He La细胞的化疗敏感性(P<0.001),这种作用可能与r Ph LTP增强顺铂诱导细胞内活性氧的产生导致细胞凋亡有关。[ Objective] To investigate the effect of recombinant Peganum harmala lipid transfer protein（rPhLTP） on the sensitivity of HeLa cells to cisplatin （DDP） chemotherapy and their possible mechanism of actions. [ Methods] The cell growth inhibitory rate was detected through MTr assay in vitro. The cell apoptosis, and the changes of ROS were measured with Annexin - V/PI staining and flow cytometry, respectively. [ Results ] Compared with individual DDP group, the proliferation inhibition rate （ P 〈 0. 01 ）, The inhibitory rate of low con- centration rPhLTP combine with 2. 5 μg/mL DDP was （42. 50 ± 0. 059）% that was much higher than the inhibitory rate of high concentra- tion DDP （10μg/mL） which rate was （36. 88 ±0. 134）%. And the apoptosis rate （P 〈 0. 01 ） were significantly enhanced when HeLa cells were treated with both rPhLTP and DDP. Also, the level of ROS generation was increased （ P 〈 0. 001 ）. [ Conclusion ] rPhLTP can induce apoptosis in HeLa cells. Combination of rPhLTP and DDP displays a synergistic effect（P 〈 0. 001 ）. The possible mechanism might be that rPhLTP induce more intracellular ROS generation,which lead to cell apoptosis.

关 键 词：重组骆驼蓬脂转移蛋白 顺铂 宫颈癌细胞 细胞凋亡 ROS活性氧 

分 类 号：R737.33[医药卫生—肿瘤]