Polydopamine-mediated immobilization of phenylboronic acid on magnetic microspheres for selective enrichment of glycoproteins and glycopeptides  

作　　者：Jiangnan Zheng Zian Lin Lan Zhang Huanghao Yang 

机构地区：[1]Key Laboratory of Analysis and Detection for Food Safety,Ministry of Education [2]Key Laboratory of Analysis and Detection Technology for Food Safety of Fujian Province [3]College of Chemistry, Fuzhou University

出　　处：《Science China Chemistry》2015年第6期1056-1064,共9页中国科学（化学英文版）

基　　金：financial support from the National Natural Science Foundation of China(21005018,21375018 and 21075016);the National Basic Research Program of China(2010CB732403);the National Science Foundation for Fostering Talents in Basic Research of China(No.J1103303);the Doctoral Fund of Ministry of Education(20103514120002);the Program for Changjiang Scholars and Innovative Research Team in University(IRT1116)

摘　　要：In this work, we demonstrate for the first time, a method to synthesize phenylboronic acid-Fe304@polydopamine （Fe3O4@ PDA-PBA） magnetic microspheres via the combination of mussel-inspired polydopamine coating and click chemistry. Uniform-size and core-shell structured Fe3O4@PDA-PBA magnetic microspheres with a core diameter of -240 nm and a shell thickness of -13 nm were obtained as identified by the characterization of the morphology, structure and composition of the synthesized microspheres. We evaluated the selectivity and binding capacity of the Fe3O4@PDA-PBA magnetic microsphcres by using standard glycoproteins （ovalbumin, immunoglobulin G and catalase） and nonglycoproteins （human serum albumin, bovine hemoglobin, myoglobin, lysozyme, and ribonuclease A） as model proteins. Adsorption experiments, SDS-PAGE and mass spectrometry analysis demonstrated that the Fe3O4@PDA-PBA magnetic microspheres had much high binding capacity and selectivity for glycoproteins/glycopeptides compared to nonglycoproteins/nonglycopeptides. In addition, the practicability of the Fe3O4@PDA-PBA magnetic microspheres was further assessed by selective capture of glycoproteins from healthy hu- man serum. The good results demonstrated its potential in glycoproteome analysis.在这个工作，我们第一次示威，综合的一个方法 phenylboronic acid-Fe <sub>3</sub > O ₄@polydopamine (Fe <sub>3</sub > O ₄@PDA-PBA) 经由启发贻贝的 polydopamine 的联合的磁性的 microspheres 涂层和按化学。一致尺寸并且核心壳组织了 Fe <sub>3</sub > O ₄@PDA-PBA 有 240 nm 的一条核心直径和 13 nm 的壳厚度的磁性的 microspheres 是由综合 microspheres 的形态学，结构和作文的描述识别了被获得。我们评估了选择和 Fe <sub>3</sub 的有约束力的能力 > O ₄@PDA-PBA 由把标准 glycoproteins (ovalbumin，免疫球蛋白 G 和过氧化氢酶) 和 nonglycoproteins (人的浆液白朊，牛的血红素，肌球素，溶解酵素，和 ribonuclease A ) 用作模型蛋白质的磁性的 microspheres。吸附实验， SDS 页和集体 spectrometry 分析证明 Fe <sub>3</sub > 磁性的 microspheres 有的 O ₄@PDA-PBA 为与 nonglycoproteins/nonglycopeptides 相比的 glycoproteins/glycopeptides 的高有约束力的能力和选择。另外， Fe <sub>3</sub 的有实行可能 > O ₄@PDA-PBA 磁性的 microspheres 被 glycoproteins 的选择俘获进一步从健康人的浆液估计。好结果在 glycoproteome 分析表明了它的潜力。

关 键 词：magnetic microspheres click chemistry phenylboronic acid ENRICHMENT GLYCOPROTEINS GLYCOPEPTIDES 

分 类 号：O652.6[理学—分析化学] TQ460.1[理学—化学]