过氧化氢通过内质网应激信号通路对Hela细胞自噬和凋亡的诱导作用  

Introduction effect of hydrogen peroxide on autophagy and apoptosis in Hela cells through endoplasmic reticulum stress signal pathway

作　　者：于洋[1] 徐冶[1] 刘师兵[1] 衣丹[2] 李松岩[1] 王晓军[1] 

机构地区：[1]吉林医药学院医学科研实验室,吉林吉林132013 [2]吉林省吉林中西医结合医院体检中心,吉林吉林132010

出　　处：《吉林大学学报（医学版）》2015年第3期437-441,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金面上项目资助课题(81372793);吉林省科技厅自然科学基金资助课题(201015240);吉林省教育厅"十二五"科研项目资助课题(吉教科合字[2013]第361号;[2014]第552号)

摘　　要：目的:利用过氧化氢(H2O2)构建人宫颈癌Hela细胞体外氧化应激模型,观察其对Hela细胞自噬和凋亡的影响,并探讨H2O2与内质网应激(ERS)信号通路之间的关系。方法:以1mmol·L-1 H2O2构建体外氧化应激模型;实验分为对照组、H2O24h组、H2O28h组和H2O212h组,各组细胞分别以H2O2作用0、4、8及12h后,利用Western blotting法检测各组Hela细胞中ERS及凋亡、自噬相关蛋白表达。结果:与对照组比较,各H2O2组Hela细胞中Cleaved Caspase-3表达水平升高(P<0.05),Cleaved Caspase-4表达水平亦明显升高(P<0.05);各H2O2组Hela细胞中LC3-Ⅱ表达水平高于对照组(P<0.05),GRP78表达水平亦高于对照组(P<0.05);随着H2O2作用时间的延长,各H2O2组Hela细胞中IRE1a表达水平明显升高(P<0.01),p-JNK表达水平明显高于对照组(P<0.05)。结论:在H2O2所构建的体外氧化应激模型中H2O2能够诱导Hela细胞发生自噬和凋亡,且该诱导作用由ERS信号通路介导。Objective To establish a superoxiaized model in vitro by using H2O2 in Hela cells and to observe the effect of H2O2 on the autophagy and apoptosis in Hela cells,and to investigate the relationship between H2O2 and endoplasmic reticulum stress（ERS）signal pathway.Methods 1 mmol·L^-1 H2O2 was employed to establish in vitro oxidative stress model.The experiment included control group,H2O2 4hgroup,H2O2 8hgroup,and H2O212hgroup;the Hela cells in various groups were treated with H2O2 for 0,4,8,and 12 h,respectively.The expression levels of apoptotic and autophagic proteins in the Hela cells in various groups were detected by Western blotting method.Results Compared with control group,the expression levels of Cleaved Caspase-3in H2O2 groups were significantly increased（P〈0.05）,and the expression levels of Cleaved Caspase-4were increased also（P〈0.05）.The expression levels of LC3-Ⅱin H2O2 groups were also increased significantly compared with control group（P〈0.05）.The expression levels of GRP78 in H2O2groups were increased compared with control group（P〈0.05）,and the expression levels of IRE1a（P〈0.01）and p-JNK（P〈0.05）were higher than those in control group.Conclusion H2O2 can induce the autophagy and apoptosis in Hela cells of in vitro oxidative stress model and its possible mechanism is related to ERS signal pathway.

关 键 词：过氧化氢 氧化应激 内质网应激 细胞凋亡 自噬 HELA细胞 

分 类 号：R737.3[医药卫生—肿瘤] R329.25[医药卫生—临床医学]