重组pIRES-AD7c-NTP质粒的构建表达及其对PC12细胞凋亡的影响  

Construction of pIRES-AD7c-NTP expression plasmid and its effect on apoptosis of cell PC12

作　　者：白春艳[1] 孙宏侠[1] 胡轶虹[1] 刘敏[1] 周艳[1] 李宗树[1] 张明明[1] 王凯[1] 

机构地区：[1]吉林省人民医院神经内科,吉林长春130021

出　　处：《中风与神经疾病杂志》2015年第5期423-426,共4页Journal of Apoplexy and Nervous Diseases

基　　金：吉林省科委课题(200705464)

摘　　要：目的构建重组pIRES-AD7c-NTP质粒,探究其对PC12细胞凋亡的影响。方法构建pIRESAD7c-NTP重组质粒,通过脂质体体外转染至PC12细胞内,RT-PCR检测凋亡相关蛋白bcl-2和bax的mRNA表达变化含量变化。结果成功构建pIRES-AD7c-NTP重组质粒并转染至PC12细胞内;重组质粒转染组出现了明显的细胞凋亡,流式细胞仪检测出典型凋亡峰;重组质粒转染组中bcl-2表达低于对照组,bax表达高于对照组。结论 AD7c-NTP重组质粒转染组PC12细胞内bcl-2/bax表达异常,说明AD7c-NTP增加凋亡促进蛋白的表达、同时减少凋亡抑制蛋白的表达,可见AD7c-NTP可导致神经细胞发生凋亡,为后续研究提供实验基础。Objective To construct pIRES-AD7c-NTP plasmid, investigate the effect on apoptosis of cell PC12. Methods The recombinant plasmids pIRES-AD7c-NTP was constructed and studied in PC12 cell. The mRNA levels of apoptosis-related protein bcl-2 and bax were measured by RT-PCR. Results We found that recombinant plasmid pIRES- AD7c-NTP was involved in the apoptosis of PC12 cells, we also observed obvious apoptosis,lower bel-2 expression and high- er bax expression. Conclusions AD7c-NTP recombinant plasmid was constructed and that the expression of bcl-2/bax was abnormal in PC12 cells, apoptosis promoting protein was raisedand anti-apoptosis protein was reduced by AD7c-NTP. AD7c-NTP leads nerve cell to apoptosis.

关 键 词：阿尔茨海默病相关神经丝蛋白 质粒构建 PC12细胞 凋亡 

分 类 号：Q51[生物学—生物化学] R749.16[医药卫生—神经病学与精神病学]