老鹰茶总黄酮和总多酚含量动态变化及抗氧化活性研究  

Study on the Dynamic Changes of the Content of Total Flavonoids and Total Polyphenol and the Antioxidant Activity of Litsea coreana Levl. var. Lanuginosa

作　　者：肖璇[1,2] 彭勇[1,2] 许利嘉[1,2] 胡华刚[3] 张鑫瑶[1,2] 肖培根[1,2] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]国家教育部中草药物质基础与资源利用重点实验室,北京100193 [3]中国中医科学院中医基础理论研究所,北京100700

出　　处：《世界科学技术-中医药现代化》2015年第3期603-608,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(81274188):四种别样茶改善胰岛素抵抗的物质基础和作用机制研究;负责人:许利嘉;北京协和医学院研究生创新基金项目(20121007024):老鹰茶改善胰岛素抵抗的物质基础和作用机理研究;负责人:肖璇

摘　　要：目的:探讨老鹰茶总黄酮和总多酚含量及抗氧化活性随季节变化的规律。方法:3月至8月定期采集老鹰茶样本,分别以芦丁和没食子酸为对照品,用紫外分光光度法分别在510 nm和753 nm波长下测定样品溶液吸光度,计算总黄酮和总多酚的含量。测定不同月份的老鹰茶对DPPH自由基的清除率,计算不同月份老鹰茶样品的EC50值,并用SPSS软件计算老鹰茶总黄酮、总多酚含量与EC50值之间的Pearson相关性。结果:3-5月份的老鹰茶总黄酮含量(17.96%-21.81%)高于6-8月份的含量(12.05%-13.64%),其中5月份的老鹰茶总黄酮含量最高;3-5月份的老鹰茶总多酚含量(8.03%-8.15%)高于6-8月份的含量(5.96%-6.16%),其中3月份的老鹰茶总多酚含量最高;3月份老鹰茶醇提物的抗氧化活性最好(EC50 5.293μg·m L-1),随着月份的累积活性降低;老鹰茶的DPPH自由基清除活性和总黄酮含量之间无显著相关性,与总多酚的含量具有显著相关性(P<0.05)。结论:总多酚是老鹰茶中主要的抗氧化活性物质,建议老鹰茶的采集时间在每年的3-5月,其中以3月最佳。The changes of total flavonoids and total polyphenol content in the Hawk Tea （Litsea coreana Levi. var. lanuginosa） and its antioxidant activity with the seasons was studied. The absorbance readings of the Hawk Tea samples, which were collected from March to August regularly, were taken at 510 nm and 753 nm after colored, respectively. And then, the content of total flavonoids and total polyphenol was calculated by comparison with a standard curve generated by analyzing rutin or gallic acid. At the same time, the ECs0 value of the Hawk Tea samples in different months were ealeulated after determination of its DPPH free radical clearance rate. Finally, The Pearson correlation among total flavonoids, total polyphenol and EC50 value were calculated using SPSS software. The content of total flavonoids in the Hawk Tea from March to May （17.96%-21.81%） was higher than tnat from June to August （12.05%-13.64%）, and in May was the highest. The total polyphenol content of the Hawk Tea from March to May （8.03%-8.15%） was higher than that from June to August （5.96%-6.16%）, and in March was the highest. The antioxidant activity of the Hawk Tea in March （5.293 μg mL-1） was best, and the activity decreased from March to August. There is no significant relevance between EC50 value and total flavonoids content in the Hawk Tea, but significant negative relevance between EC50 value and the total polyphenol content of the Hawk Tea （P〈0.05）. Total polyphenol is the main antioxidant material in the Hawk Tea. Therefore, the period from March to May each year was recommended as the harvest time and the best month is March.

关 键 词：老鹰茶 黄酮 多酚 抗氧化 

分 类 号：R282.6[医药卫生—中药学]