他米巴罗汀对牙龈卟啉单胞菌抗原刺激RAW264.7细胞的影响  

Effect of Tamibarotene on P. gingivalis antigen-stimulated RAW264. 7 cells

作　　者：靳赢[1] 王智明[1] 林晓萍[1] 

机构地区：[1]中国医科大学附属盛京医院口腔科,辽宁沈阳110004

出　　处：《口腔医学》2015年第5期349-353,共5页Stomatology

基　　金：高等学校博士学科点专项科研基金(20112104110013);辽宁省科学技术计划项目(2012225015);沈阳市科技计划项目(F13-221-9-28)

摘　　要：目的探讨他米巴罗汀(Tamibarotene,Am80)对经牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)抗原刺激后的小鼠单核巨噬细胞RAW264.7的影响。方法采用P.gingivalis抗原刺激RAW264.7细胞,分别用不同浓度的Am80进行干预;相差显微镜下观察各组细胞生长情况;MTS法检测不同浓度Am80对抗原刺激RAW264.7细胞的增殖情况;酶联免疫吸附法(ELISA)检测细胞培养上清中抗炎因子IL-10的含量。结果 10 nmol/L Am80能够显著抑制P.gingivalis抗原刺激RAW264.7细胞的增殖分化(P<0.001);Am80(10 nmol/L)致抗原刺激的细胞IL-10的分泌量显著增加(P<0.05);倒置相差显微镜下观察显示Am80抑制抗原刺激细胞的增殖分化。结论 Am80(10 nmol/L)时可显著促进P.gingivalis抗原诱导小鼠巨噬细胞分泌抗炎因子IL-10,并可能由此抑制P.gingivalis抗原诱导的炎症反应。Objective To investigate the effect of Am80 on murine macrophage RAW264.7 after P. gingivalis antigen stimulation. Methods RAW264.7 cells were stimulated by P. gingivalis antigen and treated with different concentrations of Am80. Cell growth was observed in each group under a phase contrast microscope; the proliferation of antigen-stimulated RAW264.7 cells with different concentrations of Am80 were detected by MTS assay; enzyme-linked immunosorbent assay（ELISA） was used to detect anti-inflammato- ry factor levels of IL-10 in cell culture supernatant. Results I0 nmol/L Am80 significantly inhibited the proliferation and differentia- tion of P. gingivalis antigen-stimulated RAW264.7 ceils（ P 〈 0. 001 ）while Am80 （10 nmol/L） significantly increased the secretion of IL-10 by antigen-stimulated ceils （P 〈 0.05） ; inverted phase contrast microscope showed that AmS0 inhibited antigen-stimulated pro- liferation and differentiation of RAW264.7 cells. Conclusions AmS0 （ 10 nmol/L） can significantly promote P. gingivalis antigen-in- duced murine macrophages secretion of anti-inflammatory cytokine IL-10, and it may inhibit P. gingivalis antigen-induced inflammation.

关 键 词：他米巴罗汀 巨噬细胞 INTERLEUKIN-10 牙龈卟啉单胞菌 

分 类 号：R780.2[医药卫生—口腔医学]