磷酸化caveolin在内皮-单核细胞激活多肽-Ⅱ开放血肿瘤屏障中的表达水平变化  

Changes in the expression levels of phosphorylated caveolin in endothelial monocyte activating polypeptide-II-induced opening of blood-tumor barrier

作　　者：李振[1] 刘啸白[2] 刘云会[1] 薛一雪[3] 王萍[3] 刘丽波[3] 

机构地区：[1]中国医科大学附属盛京医院神经外科,辽宁沈阳110004 [2]中国医科大学,7年制96班辽宁沈阳110001 [3]中国医科大学基础医学院神经生物教研室,辽宁沈阳110001

出　　处：《现代肿瘤医学》2015年第12期1647-1651,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81372682;81372484;81272564;81272795);沈阳市科学技术计划项目(编号:F13-318-1-16;F13-318-1-19;F13-220-9-15);高等学校博士学科点专项科研基金资助项目(编号:20092104110015;20102104110009)

摘　　要：目的:检测质膜微囊结构蛋白caveolin-1和caveolin-2在低剂量内皮-单核细胞激活多肽-Ⅱ(EMAP-Ⅱ)开放血肿瘤屏障(BTB)过程中的磷酸化水平变化。方法:荷瘤Wistar大鼠被随机分成4组(每组12只):EMAP-Ⅱ0h、1h、2h和4h组。采用伊文思蓝渗透性实验评估各组BTB通透性变化情况;Western blot和免疫荧光法检测大鼠脑胶质瘤组织毛细血管上磷酸化caveolin-1和caveolin-2的表达水平变化。结果:EMAP-Ⅱ作用1h时,BTB的通透性显著增强,随后逐渐降低,4h时恢复正常;EMAP-Ⅱ作用1h时,脑胶质瘤组织毛细血管上磷酸化caveolin-1和caveolin-2的表达水平均显著增高,随后逐渐降低,4h时恢复正常。结论:EMAP-Ⅱ可能通过caveolae介导的跨细胞途径来增强BTB的通透性,其机制与磷酸化caveolin-1和caveolin-2的表达水平上调有关。Objective：To detect changes in the expression levels of phosphorylated caveolin （p- caveolin） -1 and - 2 in low - dose endothelial monocyte activating polypeptide - U （ EMAP - I1 ） - induced opening of blood - tumor barrier（BTB）. Methods：Tumor - bearing rats were randomly divided into 4 groups（ n = 12, each） ： EMAP - II Oh, l h,2h and 4h groups. Evans blue method was used to evaluate the alterations in BTB permeability. Changes in the expression levels of p - caveolin - 1 and - 2 in rat brain capillaries were measured by Western blot and immuno- fluorescent assays. Results：The EB content in rat brain tumor tissues increased significantly at lh after EMAP- Ⅱ administration,and trended back toward the level of the EMAP - Ⅱ Oh group gradually by 4h. The expression levels of both p- caveolin- 1 and -2 in rat brain capillaries were significantly increased at l h, and decreased gradually to- ward the untreated level thereafter. Conclusion：EMAP - Ⅱ may induce opening of the BTB via caveolae - mediated transcellular pathway ,which is associated with the upregulation of p - caveolin - 1 and -2.

关 键 词：内皮-单核细胞激活多肽-Ⅱ 血肿瘤屏障 跨细胞途径 CAVEOLIN 磷酸化 

分 类 号：R730-231[医药卫生—肿瘤]