一株产赭曲霉毒素A黑曲霉的筛选与鉴定  

Screening and identification of an ochratoxin A-producing Aspergillus niger strain

作　　者：张健[1,2] 王小霞 张颖 高强[1] 

机构地区：[1]工业发酵微生物教育部重点实验室天津科技大学生物工程学院,天津300457 [2]天津实发中科百奥工业生物技术有限公司,天津300462 [3]食品营养与安全教育部重点实验室天津科技大学食品工程与生物技术学院,天津300457

出　　处：《微生物学通报》2015年第6期1010-1016,共7页Microbiology China

基　　金："十二五"国家科技支撑计划项目(No.2012BAK17B11);国家自然科学基金项目(No.31201354;31471725;31370075);国家863计划项目(No.2012AA021302)

摘　　要：【目的】筛选出可产生赭曲霉毒素A(OTA)的霉菌菌株。【方法】利用CYA和YES培养基从实验室32个霉菌样品中筛选目的菌株。利用高效液相色谱-荧光检测法对OTA产生菌进行初筛,利用高效液相色谱-质谱联用对OTA初筛菌株进行复筛。通过菌落形态、菌丝及分生孢子形态、ITS DNA序列、β-Tubulin基因序列及Calmodulin基因序列分析等鉴定目的菌株。【结果】得到一株OTA产生菌株1062,该菌株能在25°C条件下,在CYA、YES和CA培养基中很好生长。结合形态学、对培养基的要求以及上述3个基因序列的进化树分析,该菌株属于黑曲霉(Aspergillus niger)。【结论】菌株1062具有OTA产生能力,是一株黑曲霉。[Objective] The purpose of this study is to screen the fungus strain that can produce ochratoxin A（OTA）. [Methods] To this end, 32 fungal samples in our laboratory were cultivated in CYA and YES media, and screened using the high performance liquid chromatography with fluorescence detection, then rescreened using high performance liquid chromatography-mass spectrometry; According to the colony morphology, mycelial and conidia morphology as well as nucleotide sequence analysis of ITS, β-Tubulin gene and Calmodulin gene, the target strain was identified. [Results] One strain which could grow well in CYA, YES and CA media at 25 ℃was selected and named as strain 1062. Strain 1062 was identified as Aspergillus niger species by cultural and morphological characteristics and phylogenetic analysis based on ITS r DNA, β-tubulin and Calmodulin gene sequences. [Conclusion] The strain 1062 is A. niger which can produce OTA.

关 键 词：赭曲霉毒素A 黑曲霉 高效液相色谱 质谱 

分 类 号：Q93[生物学—微生物学]