EDTA预处理硬化牙本质前后的电镜观察  

Observation of the Ultrastructural Changes of Sclerotic Dentin Pretreated with EDTA by SEM

作　　者：李婷婷[1,2] 孙敏敏[1,2] 康钧棠[1,2] 夏文薇[1,2] 

机构地区：[1]上海交通大学医学院附属第九人民医院牙体牙髓科 [2]上海市口腔医学重点实验室,上海200011

出　　处：《口腔医学研究》2015年第5期506-507,510,共3页Journal of Oral Science Research

基　　金：上海交通大学医学院附属第九人民医院院基金(院2011-09)

摘　　要：目的:通过扫描电镜观察硬化牙本质经乙二胺四乙酸(ethylene diamine tetraacetic acid,EDTA)预处理前后的差异,在超微结构下分析硬化牙本质的结构改变,为临床应用EDTA预处理法提供理论依据。方法:选择10颗具有硬化牙本质(视觉分类Ⅲ级及以上)的离体牙,经扫描电镜观察比较EDTA预处理前后硬化牙本质表面超微结构的变化。结果:扫描电镜所拍摄的图片显示,经EDTA预处理后硬化牙本质表面的超矿层以及接近牙本质小管开口5μm内的钙盐沉积物全部或大部分溶解,且不破坏管口处的管周牙本质,而在牙本质小管深部,可见到较小的散在晶体。结论:EDTA能有效清除溶解牙本质小管内的矿盐沉积晶体,改善硬化牙本质的不良粘结表面,提高粘结修复的成功率。Objective：To evaluate the ultrastructure changes of sclerotic dentin in non-carious cervical lesions before and after pretreated with EDTA（Ethylene Diamine Tetraacetic Acid）.Methods：10teeth with scale Ⅲ or Ⅳsclerotic dentin were selected.The ultrastructural changes of them before and after pretreated with EDTA were observed by SEM.Rusults：After pretreated the sclerotic dentin by EDTA,the top dentinal tubules of the calcium salt deposits were completely dissolved,and there still has residual dissolved calcium salt particle in the deep dentinal tubules.Conclusion：EDTA could improve the poor adhesion of the sclerotic dentin surface and increase the bond strength.

关 键 词：硬化牙本质 乙二胺四乙酸 扫描电镜 

分 类 号：R781[医药卫生—口腔医学]