侧柏内生真菌G21代谢产物稳定性及发酵条件优化  

Metabolites Stability and Fermentation Condition Optimization of Endophytic Fungus G21 from Platycladus orientalis

作　　者：郝双红[1] 王绪昆 殷培军[2] 张相飞[1] 王栋[1] 

机构地区：[1]青岛农业大学化学与药学院山东省农业仿生应用工程技术研究中心,山东青岛266109 [2]黄岛出入境检验检疫局,山东青岛266555

出　　处：《农药》2015年第6期414-417,共4页Agrochemicals

基　　金：山东省自然科学基金(ZR2011CL007);青岛农业大学高层次人才启动基金(611001)

摘　　要：[目的]了解侧柏内生真菌G21代谢产物的稳定性,并对其发酵条件进行优化。[方法]不同p H值（2~12）或不同温度（20~120℃）处理G21发酵液,并利用生长速率法测定其抑菌活性变化。采用正交试验法及响应面法对G21发酵条件进行优化。[结果]G21发酵液具有广谱农用抗真菌活性,对苹果腐烂病菌及葡萄白腐病菌抑制作用显著;其发酵液在中性（p H 6~8）及较低温度（50℃）下稳定。优化G21发酵培养基组成为蛋白胨3.1 g/L、葡萄糖11.8 g/L、硫酸镁0.58 g/L、磷酸二氢钾1.0 g/L。[结论]G21发酵液处理过程应避免酸碱及高温,优化得培养基G21发酵产物抑菌活性显著提高。[Aims] The stability of metabolites of endophytic fungus G21 from P. orientalis and its fermentation conditions were studied. [Methods] The fermentation broth of G21 was treated under different pH （2-12） or temperature （20-120℃ ）, then their antifungal activity against Valsa mali and Coniothyrium diplodieUa were evaluated by growth rate method. Orthogonal experiment and response surface methodology were applied to optimize the fermentation conditions of G21. [Results] The fermentation broth of G21 exhibited broad antifungal spectrum, and could remarkably inhibited the mycelium of V. mali and C. diplodiella. The fermentation broth was stable at pH 6-8 and below 50 ℃. The optimal constituents of the fermentation medium for G21 were peptone 3.1 g/L, glucose 1 t.8 g/L, magnesium sulfate 0.58 g/L, monopotassium phosphate 1.0 g/L. [Conclusions] Acid, alkali or high temperature should be avoided during the treatment process for the fermentation broth. The antifungal activity of the fermentation broth in optimized fermentation medium was significantly increased.

关 键 词：内生真菌 抑菌活性 发酵培养基 响应面法 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]