黄色短杆菌产L-精氨酸发酵培养基的优化  

Optimization of Fermentation Medium for L-Arginine Production with Brevibacterium Flavum

作　　者：赵鑫[1,2] 肖玉平[1] 李立英[1] 

机构地区：[1]广东轻工职业技术学院食品与生物工程系,广东广州510300 [2]广东高校特色调味品工程技术开发中心,广东广州510300

出　　处：《食品研究与开发》2015年第10期110-114,共5页Food Research and Development

摘　　要：在液体培养条件下,采用单因素试验法,考察了发酵培养基中不同浓度的碳源、氮源、无机盐和生物素对实验室筛选获得的黄色短杆菌L-Arg高产突变株GC 1325产酸的影响,确定了碳源和氮源的初始浓度和补加方式。实验结果表明,GC 1325发酵的最优培养基为:葡萄糖100 g/L,(NH4)2SO425 g/L、KH2PO41.2 g/L、Mg SO4·7H2O 0.6 g/L、生物素100μg/L;发酵过程中24 h后一次流加50 g/L葡萄糖维持碳源;连续流加25%的氨水维持(NH4)2SO4浓度在25 g/L水平,在此优化的培养条件下,GC 1325获得的最大的L-Arg产量为37.8 g/L,比优化前提高了58.8%。At the liquid culture conditions, the fermentation medium of GC 1325, the high yielding of L-arginine strain mutagenized form brevibacterium flavum optimized using single factor test. Different concentrations of carbon sources, nitrogen sources, inorganic salts, and thiamine effect on the production of L-arginine were studied. The initial concentration of carbon sources , nitrogen sources and additional way were confirmed. The experimental results show that the optimal fermentation medium for GC 1325：glucose 100 g/L, （NH4）2SO4 25 g/L,KH2PO4 1.2 g/L,MgSO4·7H2O 0.6 g/L, biotin 100μg/L;Glucose （50 g/L） fed once at 24 h and 25 % aqueous ammonia fed continuously to maintain the （NH4）2SO4 concentration was 25 g/L in the fermentation process. At this optimization of culture conditions, the maximum yield of L-arginine was 37.8 g/L, which was increased by 58.8%.

关 键 词：黄色短杆菌 发酵培养基 优化 L-精氨酸 

分 类 号：TQ922[轻工技术与工程—发酵工程]