EPHB6的缺失突变del915-917促进非小细胞肺癌细胞的迁移  

Deletion mutation of EPHB6 del915-917 enhances migration of non-small cell lung cancer cells

作　　者：于军[1] 铁茹[1] 张学策[1] 常盼[1] 赵鸽[2] 陈宝莹[3] 

机构地区：[1]西安医学院第二附属医院中心实验室,西安710038 [2]西安交通大学医学院第一附属医院麻醉科 [3]第四军医大学唐都医院放射科

出　　处：《山西医科大学学报》2015年第6期539-543,共5页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(30801385);卫生部肿瘤个体化治疗分子诊断专项基金资助项目(W2013FZ20);陕西省社会发展公关基金资助项目(2014K11-01-01-07);陕西省教育厅基础研究专项基金资助项目(14JK1616)

摘　　要：目的明确非小细胞肺癌(non-small cell lung cancer,NSCLC)中EPHB6的缺失突变del915-917的功能性作用。方法定点突变EPHB6的表达载体pc DNA4-EPHB6-wt获得突变载体pc DNA4-EPHB6-mut,进而转染肺腺癌细胞A549,检测细胞的迁移、划痕愈合、细胞增殖活性和细胞大小。结果 EPHB6的缺失突变del915-917增强NSCLC细胞体外迁移,加速划痕愈合。不改变细胞的增殖活性,但明显使细胞变小。结论 EPHB6的缺失突变del915-917通过改变细胞大小而增强NSCLC细胞的迁移能力。Objective To identify the functional consequences of de1915-917, the EPHB6 mutation, in non-small cell lung cancer cells. Methods Site-directed mutation was performed from pcDNA4-EPHB6-wt to pcDNA4-EPHB6-mut, and then transfected into A549 lung adenocarcinoma cells. The in vitro migration and scratch assays were performed. Also, the proliferation and size of A549 ceils were evaluated. Results The deletion mutation de1915-917 enhanced the migration and accelerated the wound healing in vitro. The proliferation of A549 cells was not affected by EPHB6 mutation of de1915-917. However, the size of A549 cells was decreased in the A549 cells expressing de1915-917. Conclusion De1915-917, the deletion mutation of EPHB6, promotes the migration of NSCLC by altering the cell size.

关 键 词：EPHB6 迁移 受体型酪氨酸蛋白激酶 突变 

分 类 号：R734.2[医药卫生—肿瘤]