全水化无醇提取γ-聚谷氨酸工艺的研究  

Study on the separation and purification of poly- γ- glutamtic acid without any organic solvents

作　　者：乔长晟[1,2] 张苗苗[1] 刘晓晨[3] 高明昊[1] 林虹秀 楼鹏 

机构地区：[1]天津科技大学工业微生物教育部重点实验室,天津300457 [2]天津北洋百川生物技术有限公司,天津300457 [3]食品营养与安全省部共建教育部重点实验室,天津300457

出　　处：《食品工业科技》2015年第13期247-251,共5页Science and Technology of Food Industry

摘　　要：为实现γ-聚谷氨酸无醇提取,利用板框除菌,酶解除大分子多糖,活性炭脱色,超滤除小分子杂质,最后冷冻干燥获得γ-聚谷氨酸(γ-PGA)成品。确定了全水化无醇提取条件:下罐调p H6.0,发酵液稀释2倍;硅藻土和红土配比为2∶1(m∶m),总添加量为1.5%(w/v),除菌率97%以上;脱色条件:活性炭添加量1.5%(w/v)、温度30℃、p H5、时间60min。超滤选择10ku的膜包除杂并最终浓缩至原液的1/9。冷冻干燥18h得到的γ-聚谷氨酸纯度达90%。无醇提取工艺的探索为工业生产提取γ-PGA提供了重要参考依据。Abstract.For separating γ-PGA without any alcohol, a extractive method was developed to separate and purify γ-PGA from culture broth.The culture broth was filtrated with diatomite to remove cells, and the macromolecular polysaccharide was catabolized by enzymolysis.Then,active carbon was added as decoloring agent and the small molecular weight impurity was removed by ultrafiltration.Finally, purified γ-PGA was obtained by lyophilization.The optimum quantity of using mixed diatomite loading（ ratio of coarse to small diatomite 2：1 ）was 1.5% （w/v）and over 97% of cells were removed from y-PGA broth.The optimum conditions of decoloration were as follows.the addition of active carbon 1.5% （w/v）, temperature 30℃, pHS, processing time 60min.The optimum membrane module of ultrafiltration was lOku and the concentration rate of ultrafiltration was 9.1.The purity of γ- PGA was 90% after lyophilization for ]Sh.The study of separation and purification of poly-y-glutamtic acid without any organic solvents was imperative and it provided an important basis for Industrial production of γ-PGA.

关 键 词：Γ-PGA 除菌 无醇提取 脱色 纯化 

分 类 号：TS201.2[轻工技术与工程—食品科学]