饲喂黄曲霉毒素B_1对奶山羊乳中黄曲霉毒素M_1分泌规律与转化率的影响  

Excretion Pattern and Carry-Over Rate of Aflatoxin M_1 in Milk of Dairy Goats after Fed Aflatoxin B_1

作　　者：王丽芳[1] 姚一萍[1] 连海飞[1] 张欣昕[1] 李秀萍[1] 曾杰[1] 

机构地区：[1]内蒙古农牧业科学院资源环境与检测技术研究所,呼和浩特010031

出　　处：《动物营养学报》2015年第6期1915-1920,共6页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家农产品质量安全风险评估重大专项(GJFP2014008);内蒙古农牧业科学院创新基金项目;内蒙古自治区人才开发基金项目

摘　　要：为研究饲粮中添加单一剂量黄曲霉毒素 B1（ AFB1）后奶山羊乳中黄曲霉毒素 M1（AFM1）的分泌规律及AFB1转化为AFM1的转化率,选择3只健康、体重接近的泌乳中期关中奶山羊,每只羊饲喂1 mg AFB1。饲喂后0、4、8、24、32、48、56、72、80、96、104及120 h采集羊乳,测定AFM1含量。结果表明：1）饲喂AFB1前（0 h）,羊乳中未检出AFM1,但是饲喂AFB1后4 h,在收集的羊乳中均检出了AFM1；2）3只试验在羊乳中AFM1含量存在差异,但是其分泌规律非常相似,均呈先升高后急剧下降再缓慢下降的趋势；3）羊乳中 AFM1的峰值出现在饲喂AFB1后4～8 h；4）羊乳中 AFM1平均含量在56 h 时为0.39μg/kg,低于我国限量值（0.5μg/kg）,到120 h平均含量为0.02μg/kg,低于欧盟限量值（0.05μg/kg）；5）各时间点AFB1转化为AFM1的最大平均转化率为0.19%,出现在4 h；6）饲喂AFB1后24 h内的AFM1平均转化率之和占AFM1总转化率的81%。结果提示,AFM1在奶山羊乳中的排出时间主要集中在采食AFB1后的24 h内。The aim of this study was to determine the excretion patterns and carry-over rate of aflatoxin M1 （AFM1） in milk of dairy goats fed a single dose of aflatoxin B1（AFB1）. Three healthy Guanzhong dairy goats in mid-lactation with similar body weight were administered 1 mg of AFB1. Milk samples were collected after 0, 4, 8, 24, 32, 48, 56, 72, 80, 96, 104 and 120 h of AFB1 administration to determine AFM1 content. The results showed as follows：1） AFM1 was not detected in milk before AFB1 administration （0 h）, but was detected in the first milking following AFB1 administration （4 h）;2） the excretion pattern of AFM1 content in milk was very similar in all the experimental goats even if the values differed among animals;3） The peak val-ue for AFM1 content in milk was observed in milk collected at 4 and 8 h after AFB1 administration;4） AFM1 average content in milk was 0.39 μg/kg at 56 h and 0.02 μg/kg at 120 h, which moved below the Chinese limit （0.5 μg/kg）and the European Union limit （0.05 μg/kg）, respectively;5） the maximum value of aver-age carry-over rate of AFB1 into AFM1 in milk was 0.19% which appeared at 4 h;6） the sum of average car-ry-over rate at different time point in 24 h was 81% of total average carry-over rate after AFB1 administration. In conclusion, AFM1 is mostly excreted in milk of dairy goats in 24 h after AFB1 administration.

关 键 词：奶山羊 黄曲霉毒素B1 羊乳 黄曲霉毒素M1分泌规律 转化率 

分 类 号：S826[农业科学—畜牧学]