UV-C对蓝莓酚类物质及其相关酶活性的影响  

Effect of UV-C treatment on phenolic compounds and relevant enzymes activities of blueberry

作　　者：杨乐[1] 侯智霞[1] 杨俊枫 宫中志 王冲[1] 史文君[1] 

机构地区：[1]北京林业大学省部共建森林培育与保护教育部重点实验室,北京100083

出　　处：《浙江农业学报》2015年第6期955-960,共6页Acta Agriculturae Zhejiangensis

基　　金：中央高校基本科研业务费专项资金资助项目(YX2013-12)

摘　　要：为了探究UV-C照射处理对蓝莓不同发育期果实的酚类物质及相关酶活的影响,以品种北陆为试材,辐照时间分别为5,10,15 min,以不照射为对照。处理后避光贮存于人工气候箱(温度23℃,相对湿度95%)中,24 h后取样分析果实中总酚、类黄酮含量、苯丙氨酸解氨酶(PAL)和查尔酮异构酶(CHI)活性的变化。研究结果表明,UV-C处理能促进不同发育时期蓝莓果实总酚和类黄酮的积累,显著降低幼果CHI活性,增加其余发育时期果实的PAL及CHI活性,且照射10 min的剂量效果最为显著。PAL与CHI活性均与总酚和类黄酮的积累呈正相关。The effect of UV-C treatment on the phenolic compounds and relevant enzymes activities of blueberry during fruit development was conducted. The radiation dose was 5,10,15 min,taking no irradiation as the control. The results showed that UV-C irradiation could promote the accumulation of total phenols and flavonoids during development stage,decreased the CHI activity in young fruits,increased the PAL and CHI activity in other stages. And the effect of 10 min irradiation was the most significant. The activity of PAL and CHI were positively correlated to total phenol and flavonoids accumulation.

关 键 词：蓝莓 总酚 类黄酮 PAL活性 CHI活性 UV-C 

分 类 号：S663.9[农业科学—果树学]