电针百会穴对APP/PS1双转基因小鼠学习记忆及脑源性神经营养因子表达的影响  

Effects of Electroacupuncture at Baihui(DU20) on Learning and Memory and Expression of Brain-derived Neurotrophic Factor in APP/PS1 Double-transgenic Mice

作　　者：陈吉祥[1] 吴羽楠 郑雅媗[2] 卓沛元[1] 张颖铮 陈立典[1] 

机构地区：[1]福建中医药大学康复医学院,福建福州市350122 [2]福建中医药大学药学院,福建福州市350122

出　　处：《中国康复理论与实践》2015年第6期642-647,共6页Chinese Journal of Rehabilitation Theory and Practice

基　　金：福建省康复技术协同创新中心资助项目(No.X2012004-协同)

摘　　要：目的观察电针百会穴对APP/PS1双转基因小鼠学习记忆功能及脑源性神经营养因子(BDNF)表达的影响,探讨电针治疗阿尔茨海默病的作用机制。方法将30只4月龄雌性APP/PS1双转基因小鼠随机分为模型组、百会组和非穴组,每组10只,另取10只同窝阴性野生小鼠为野生组。百会组电针百会穴,非穴组电针非穴,干预28 d,野生组和模型组不干预。采用Morris水迷宫实验观察小鼠学习记忆能力;免疫组化技术观察小鼠大脑皮质β-淀粉样蛋白的沉积;Western blotting和RT-PCR法检测小鼠皮质脑源性神经营养因子(BDNF)蛋白和基因的表达情况。结果与模型组相比,百会组小鼠逃避潜伏期缩短(P<0.05),跨越平台次数明显增加(P<0.01);β-淀粉样蛋白的沉积减少(P<0.05);BDNF蛋白和基因的表达明显增多(P<0.01)。非穴组与模型组相比无显著性差异(P>0.05)。结论电针百会穴可改善APP/PS1双转基因小鼠的学习记忆功能,其作用机制可能与增加大脑皮质BDNF蛋白和基因的表达,降低β-淀粉样蛋白的沉积有关。Objective To explore the effects of electroacupuncture at Baihui （DU20） acupoint on learning and memory and its possible mechanism through the expression of brain-derived neurotrophic factor （BDNF） in APP/PS1 double-transgenic mice. Methods 30 female APP/PS1 double transgenic mice were randomly divided into model group, DU20 group and non-acupoint group, and 10 wild type mice con- sisted of wild group. DU20 group received electroacupuncture at Baihui and the non-acupoint group received electroacupuncturc at non-acu- point for 28 days. Learning and memory was tested by Morris water maze. Deposition of β-amyloid （Aβ） peptide was determined by immu- nohistochemical staining. The expression of BDNF in cortex was examined by RT-PCR and Western blotting. Results Compared with the model group, DU20 group ameliorated the learning and memory ability ofAPP/PS1 double-transgenic mice （P〈0.05）, decreased the deposi- tion ofA~ peptide （P〈0.05） and upregulated the gene and protein levels of BDNF （P〈0.01）. There was no significant difference between the model group and non-acupoint group （P〉0.05）. Conclusion Electroacupuncture at DU20 acupoint could ameliorate learning and memory in APP/PS 1 double-transgenic mice. The mechanism may be related to increase the expression of BDNF and decrease the deposition of Aβ.

关 键 词：电针 百会穴 APP/PS1 学习记忆 脑源性神经营养因子 小鼠 

分 类 号：R749.1[医药卫生—神经病学与精神病学]