小麦低聚肽对H_2O_2所致HepG2细胞氧化损伤的保护作用  

Protective Effect of Wheat Oligopeptides on Hydrogen Peroxide-Induced HepG2 Cell Injury

作　　者：刘艳[1] 张海欣[1] 魏颖[1] 林峰[1] 蔡木易[1] 

机构地区：[1]中国食品发酵工业研究院北京市蛋白功能肽工程技术研究中心,北京100015

出　　处：《食品与生物技术学报》2015年第6期599-604,共6页Journal of Food Science and Biotechnology

基　　金：国家"十二五"科技支撑计划项目(2012BAD33B04-02);国家863计划项目(2013AA102205-02);科技北京百名领军人才培养工程项目(Z131110000513026);北京市科技计划项目(Z131100003113010)

摘　　要：分析小麦低聚肽氨基酸组成和相对分子质量分布,并对其保护过氧化氢所致人肝癌细胞(HepG2)氧化损伤的作用进行了研究。首先采用不同浓度小麦低聚肽作用HepG2细胞4 h,然后用150μmol/L过氧化氢氧化损伤细胞4 h,以细胞存活率、细胞内活性氧(ROS)及丙二醛(MDA)含量评价小麦低聚肽对HepG2细胞的保护作用。结果表明,小麦低聚肽相对分子质量小于1 000的组分高达93.44%,谷氨酸及脯氨酸含量较高;小麦低聚肽具有增加细胞存活率、抑制细胞内ROS生成、降低细胞内MDA含量的活性,对减少HepG2细胞的氧化应激及提高细胞抗氧化能力具有较好的作用效果。本研究从细胞水平上揭示了小麦低聚肽对过氧化氢所致的肝脏损伤具有一定的预防保护作用。Amino acid composition and molecular weight distribution of wheat oligopeptides （WOP） were analyzed, and the protective effect of WOP on hydrogen peroxide （H2O2）-induced HepG2 cell oxidative injury was investigated. After treated with different concentrations of WOP for 4 h, HepG2 cells were injured by 150 μmol/L of H2O2 for 4h, and then the cell viability and contents of intracellular reactive oxygen species （ROS） and malondialdehyde （MDA） were determined. Results showed that the percentage of WOP with molecular weight below 1,000 Da reached up to 93.44%and the contents of glutamic acid, glutamine and proline were relatively high. Furthermore, WOP increased the cell viability and inhibited the production of intracellular ROS and MDA, indicating beneficial effects of WOP on the reduction of oxidative stress and the improvement of cell antioxidant capacity. Thus, WOP could protect liver cells against the injury caused by peroxides.

关 键 词：小麦低聚肽 HEPG2细胞 过氧化氢 氧化损伤 

分 类 号：S512.1[农业科学—作物学] TS201.4[轻工技术与工程—食品科学]