Box-Behnken响应面法优化葛花与枳椇子中总黄酮提取工艺的研究  

Optimization of Extraction Technology for Total Flavonoids fromFlos puerariae and Hovenia dulcis by RSM of Box-Behnken

作　　者：洪金艳 李洪军[1,2] 甘奕[1] 陈康[1] 夏启禹[1] 贺稚非[1,2] 

机构地区：[1]西南大学食品科学学院,重庆400715 [2]重庆市农产品加工技术重点实验室,重庆400715

出　　处：《西南大学学报（自然科学版）》2014年第10期186-192,共7页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金面上项目(31071566);西南大学国际合作项目(20131220)

摘　　要：利用响应面分析法对葛花与枳椇子中总黄酮的提取工艺进行优化.通过单因素试验分别考察原料比、粉碎粒度、料液比和提取时间对总黄酮得率的影响.选择适当的试验因素和水平,利用Design Expert软件和Box-Behnken设计法设计响应面试验,对各个因素的显著性以及交互作用进行分析.结果表明:葛花与枳椇子中总黄酮的提取工艺最佳条件为原料比(葛花与枳椇子)2.2∶1,不粉碎,料液比1∶21,时间85min,在该条件下验证,总黄酮得率可达到8.31mg/g,接近预测值8.37mg/g.最优提取条件下的提取次数试验表明,提取2次效果最好.Response surface analysis methodology （RSM ） was used for optimizing the extraction technolo-gy for total flavonoids from Flos puerariae and Hovenia dulcis .In single-factor tests ,raw material ratio , fineness of pulverization ,liquid-material ratio and extraction time on extraction rate were explored .A re-gression model describing extraction rate for total flavonoids as a function of three variables was estab-lished with the Design-Expert software according to the Box-Behnken design principle and experimental da-ta .The optimal extraction conditions for total flavonoids from F. puerariae and H. dulcis were raw ma-terial ratio of 2.2 ∶ 1 ,non-grinding ,material-liquid ratio of 1 ∶ 21 ,extraction time of 85 min and extrac-tion twice .Under the optimal extraction conditions ,the maximum extraction yield of total flavonoids was 8.31 mg /g ,which was close to the predicted value （8.37 mg/g） .

关 键 词：葛花 枳椇子 总黄酮 响应面分析法 提取 

分 类 号：TS251.54[轻工技术与工程—农产品加工及贮藏工程]