油桐delta 8脱饱和酶基因的全长cDNA克隆及序列分析  

Molecular cloning of full-length cDNA of delta 8 fatty acid desaturase gene from Vernicia fordii

作　　者：龙洪旭[1] 谭晓风[1] 张琳[1] 曾艳玲[1] 李泽[1] 王哲[1] 

机构地区：[1]中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南长沙410004

出　　处：《中南林业科技大学学报》2015年第7期12-16,26,共6页Journal of Central South University of Forestry & Technology

基　　金：国家林业公益性行业科研专项重大项目(201204403);湖南省研究生科研创新基金资助项目(CX2014B327)

摘　　要：桐油是制备生物柴油的优势原料。delta 8脱饱和酶基因与植物膜脂、鞘磷脂的生物合成及细胞信号传递途径有关。通过简并PCR扩增、3’RACE、5’RACE及编码区扩增获得了油桐delta 8脱饱和酶的全长c DNA序列。该基因全长1 787 bp,ORF的长度为1 344 bp,编码447个氨基酸。经过网上比对分析,发现油桐delta 8脱饱和酶与其它植物的此基因具有较高同源性,其中与蓖麻脂肪酸去饱和酶相似度达83%。研究结果为揭示油桐油脂合成途径及分子定向育种奠定基础。Tung oil of Verniciafordii is the high-quality raw material for bio-diesel. Delta 8 fatty acid desaturase gene is related with the biosynthesis of plant membrane lipids, sphingomyelin biosynthesis and the cell signaling pathway. Full-length cDNA sequence of delta 8 desaturase gene was obtained for the first time by using methods of degenerate PCR, 3＇ RACE and 5＇ RACE from maturing seed of E fordii. Gene＇s full-length is 1 787 bp and contained a 1 344 bp ORF （open reading frame）, encoding a peptide of 447amino acids. The results of comparative analysis show that delta 8 fatty acid desaturase gene from E fordii had a high similarity to those of other plants, of them, the similarity of V. fordii to Ricinus communis delta 8 gene reached 83%. The findings laya foundation for revealing the lipid synthesis pathway and molecular directed breeding.

关 键 词：油桐 DELTA 8去饱和酶 简并PCR RACE 基因克隆 

分 类 号：S794.3[农业科学—林木遗传育种]