雄激素受体基因CAG多态性对前列腺癌恶性程度的影响  

Effect of androgen receptor gene (CAG)_n polymorphism repeats on the severity of prostate cancer

作　　者：梁国庆[1] 顾燕青[2] 沈薇[3] 陈伟杰[1] 江宁东[1] 陈春雷[1] 沙建军[4] 

机构地区：[1]上海中医药大学附属市中医医院泌尿外科,上海200071 [2]上海交通大学医学院附属新华医院崇明分院泌尿外科,上海202150 [3]上海交通大学医学院附属仁济医院检验科,上海200127 [4]上海交通大学医学院附属仁济医院泌尿外科,上海200127

出　　处：《现代泌尿外科杂志》2015年第6期420-422,共3页Journal of Modern Urology

基　　金：上海市卫生局课题(No.20134392)

摘　　要：目的检测正常人群、前列腺癌（PCa）低危和高危组雄激素受体（AR）基因（CAG）n微卫星多态性,探讨该重复序列长度对PCa恶性程度的影响。方法应用PCR和双链循环DNA测序技术,对98例正常人群（对照组）和95例经前列腺穿刺活检证实为PCa的患者,进行外周血标本（CAG）n串联重复序列检测,分析该微卫星多态性与PCa恶性程度的影响。结果 PCa组CAG重复序列为15-31,平均（23.21±3.07）;参照组重复序列为14-34,平均（24.20±3.50）,两者比较差异具有显著统计学意义（P〈0.05）。根据PCa患者肿瘤Gleason评分进行分级,≤7分为低危,共68例,≥8分为高危,27例。低危组的CAG重复序列为18-31,平均（23.65±2.92）,高危组CAG重复序列为15-27,平均（22.11±3.21）,两者比较差异具有统计学意义（P〈0.05）,高危组与参照组比较差异具有显著统计学意义（P〈0.01）。结论受试者AR基因（CAG）n重复序列呈现多态性,该重复序列长度与PCa的发生有关,较短的CAG重复序列预示发生PCa的可能性较大,恶性程度更高。Objective To screen the （CAG）. polymorphism of androgen receptor （AR） gene in healthy men, patients with mild prostate cancer （PCa） and severe PCa, and to explore the relationship between the polymorphism with PCa severity. Methods A total of 98 healthy men （control group） and 95 PCa patients were enrolled. The length of AR gene CAG short tandem repeat （CAG-STR） sequences were detected by PCR and dsDNA cycle sequencing, and the effect of （CAG）.polymor- phism on PCa severity was analyzed. Results The number of CAG repeats for the PCa group and the control group were 15-31 （23.21±3.07） and 14 - 34 （24.20±3.50） respectively, with statistically significant difference between them （P 0.05）. According to PCa Gleason level, 68 cases were mild PCa （47） and 27 cases were severe PCa （98）. The number of CAG repeats for the mild and severe PCa groups were 18 - 31 （23.65±2.92） and 15 - 27 （22.11 ± 3.21 ）, respectively, with a significant difference. The number of CAG repeats in the severe PCa group was fewer than that in the control group （P〈0.01）. Conclusions The length of AR gene CAG repeat sequences present polymorphism in the subjects and may be one of the risk factors for PCa. Shorter CAG repeat sequences suggest severer PCa. KEY WORDS： genes; polymorphism; （CAG）n sequence repeat; androgen receptor; prostate cancer; severity degree

关 键 词：基因 多态性 CAG重复序列 雄激素受体 前列腺癌 恶性程度 

分 类 号：R737.25[医药卫生—肿瘤]