pilO基因对鲍曼不动杆菌运动能力的影响  

Gene pil O is Necessary for Twitching Movement of Acinetobacter baumannii

作　　者：郭通[1,2] 刘雄[2] 王德慧[2] 李崭[2] 黄洁[2] 李涛[2] 王慧[2] 

机构地区：[1]广西医科大学,广西南宁530000 [2]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071

出　　处：《生物技术通讯》2015年第3期321-324,共4页Letters in Biotechnology

基　　金：国家自然科学基金(81401643);国家重点基础研究发展计划(2015CB554202)

摘　　要：目的:构建无标记的鲍曼不动杆菌pil O基因缺失突变株,通过表型鉴定pil O基因缺失对鲍曼不动杆菌运动能力的影响。方法:PCR扩增pil O基因上下游各1 kb同源臂,连接至p MO130-TelR自杀质粒,质粒转化大肠杆菌S17-1后再接合至鲍曼不动杆菌4294中;蔗糖诱导自杀质粒与基因组同源重组,以获得基因缺失突变株;蹭行实验观察pil O基因缺失对鲍曼不动杆菌运动能力的影响。结果:构建了pil O基因缺失鲍曼不动杆菌4294菌株突变株;缺失株与野生株生长曲线无明显差异,但蹭行能力显著下降。结论:pil O基因与细菌蹭行能力密切相关,提示其编码鲍曼不动杆菌Ⅳ型菌毛结构蛋白。Objective： To construct marker-less gene pil O deleted Acinetobacter baumannii,and observe move-ment difference between wild and mutant strain through phenotype.Methods： Up and downstream homolo-gous arms about 1 kb were amplified from gene pil O by PCR,then they were inserted into the suicide plasmidp MO130-TelR.The plasmid was electrotransformed into E.coli S17-1,and then transferred into A.baumannii 4294 strain by conjugation.The sucrose was added to select the marker-less gene pil O deletion strain.The locomotivityreflecting twitching motility were compared between mutant and wild strain.Results： No growth difference was ob-served between mutant and wild strain,but twitching motility of A.baumannii 4294Δpil O decreased significantly.Conclusion： Gene pil O closely associate with twitching motility,suggesting it might encode structural protein oftype Ⅳ pili.

关 键 词：鲍曼不动杆菌 缺失株 自杀质粒 蹭行运动 

分 类 号：Q78[生物学—分子生物学]