Three-stage fermentation and kinetic modeling of bioflocculant by Corynebacterium glutamicum  

三段式谷氨酸棒杆菌发酵生产生物絮凝剂的过程及动力学模拟(英文)

作　　者：沈亮 安仲涛 李清彪 姚传义 彭雅娟 王远鹏 赖瑞华 邓旭 何宁 

机构地区：[1]Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, The Key Lab for Synthetic Biotechnology of Xiamen City [2]College of Life Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University

出　　处：《Chinese Journal of Chemical Engineering》2015年第1期219-226,共8页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(21206143,51378444);the program for New Century Excellent Talents of Education Ministry of China(ncet-13-0501)

摘　　要：Fermentation of bioflocculant with Corynebacterium glutamicum was studied by way of kinetic modeling.Lorentzian modified Logistic model, time-corrected Luedeking–Piret and Luedeking–Piret type models were proposed and applied to describe the cell growth, bioflocculant synthesis and consumption of substrates, with the correlation of initial biomass concentration and initial glucose concentration, respectively. The results showed that these models could well characterize the batch culture process of C. glutamicum at various initial glucose concentrations from 10.0 to 17.5 g·L-1. The initial biomass concentration could shorten the lag time of cell growth,while the maximum biomass concentration was achieved only at the optimal initial glucose concentration of16.22 g·L-1. A novel three-stage fed-batch strategy for bioflocculant production was developed based on the model prediction, in which the lag phase, quick biomass growth and bioflocculant production stages were sequentially proceeded with the adjustment of glucose concentration and dissolved oxygen. Biomass of2.23 g·L-1was obtained and bioflocculant concentration was enhanced to 176.32 mg·L-1, 18.62% and403.63% higher than those in the batch process, respectively, indicating an efficient fed-batch culture strategy for bioflocculant production.Fermentation of bioflocculant with Corynebacterium glutamicum was studied by way of kinetic modeling.Lorentzian modified Logistic model, time-corrected Luedeking–Piret and Luedeking–Piret type models were proposed and applied to describe the cell growth, bioflocculant synthesis and consumption of substrates, with the correlation of initial biomass concentration and initial glucose concentration, respectively. The results showed that these models could well characterize the batch culture process of C. glutamicum at various initial glucose concentrations from 10.0 to 17.5 g·L-1. The initial biomass concentration could shorten the lag time of cell growth,while the maximum biomass concentration was achieved only at the optimal initial glucose concentration of16.22 g·L-1. A novel three-stage fed-batch strategy for bioflocculant production was developed based on the model prediction, in which the lag phase, quick biomass growth and bioflocculant production stages were sequentially proceeded with the adjustment of glucose concentration and dissolved oxygen. Biomass of2.23 g·L-1was obtained and bioflocculant concentration was enhanced to 176.32 mg·L-1, 18.62% and403.63% higher than those in the batch process, respectively, indicating an efficient fed-batch culture strategy for bioflocculant production.

关 键 词：BIOFLOCCULANT FERMENTATION Corynebacterium glutamicum MODELING KINETICS 

分 类 号：TQ920[轻工技术与工程—发酵工程]