悬浮培养发状念珠蓝细菌对盐碱胁迫的生理应答研究  

Physiological Responses to Saline-alkali Stress in Nostoc flagelliforme

作　　者：郭金英[1] 朱蓓茹 吴影[1] 任国艳[1] 王萍[1] 崔国庭[1] 张玉先[1] 冯惠敏[1] 

机构地区：[1]河南科技大学食品与生物工程学院,洛阳471023

出　　处：《天然产物研究与开发》2015年第6期990-994,共5页Natural Product Research and Development

基　　金：河南省重点科技攻关计划(142102110038);河南科技大学博士科研启动基金;河南科技大学大学生研究训练(SRTP)项目(2014117)

摘　　要：天然发状念珠蓝细菌是荒漠化土壤中的"先锋生物",具有极强的耐旱、耐碱、耐高温等能力。本研究旨在揭示悬浮培养发状念珠蓝细菌的抗盐碱能力,为发状念珠蓝细菌的人工培养提供基础。25℃,80μmol/(m2·s)光照下,BG-11培养液培养发状念珠蓝细菌,利用不同浓度(0、60、120、180、240 mmol/L)混合的Na2SO4和Na2CO3胁迫发状念珠蓝细菌细胞,处理时间分别为24 h和72 h,测定其质膜透性、超氧化物歧化酶活性、丙二醛、脯氨酸、可溶性蛋白以及可溶性多糖含量,分析发状念珠蓝细菌细胞对盐碱胁迫的响应。盐碱胁迫下,随着胁迫程度的加重,发状念珠蓝细菌细胞的质膜相对透性增加,丙二醛含量上升;超氧化物歧化酶活性和脯氨酸含量先上升后下降;可溶性蛋白含量逐渐下降;可溶性多糖含量不断升高。悬浮培养的发状念珠蓝细菌细胞对盐碱胁迫产生结构和生理的应激响应,增强了发状念珠蓝细菌抗盐碱能力。Natural Nostoc flagelliforme has resistance to drought resistance,alkali,and high temperature. This study aimed to investigate the effects of saline alkali stress on N. flagelliforme cell. The modified BG-11 culture medium containing different concentrations（ 0,60,120,180,240 mmol / L） of saline-alkali was used to culture N. flagelliforme cell at25 ℃,80 μmol /（ m2·s）. Electrolyte leakage,the activities of superoxide dismutase,the content of malondialdehyde,proline,soluble protein and trehalose were determined. The response of N. flagelliforme cell was analyzed under the stress. Under saline-alkali stress,along with the stress severity,N. flagelliforme cell increased relative plasmalemma permeability and MDA content. Superoxide dismutase activity and proline content increased at first and then decreased. The content of soluble protein gradually decreased,but trehalose content increased. Saline-alkali stress can induce structure and physiological response of N. flagelliforme cell,and it could resist mild injury caused by saline-alkali.

关 键 词：盐碱 发状念珠蓝细菌 生理应答 

分 类 号：Q945.78[生物学—植物学]