响应面法优化杜香多糖提取工艺  

Optimization of Extraction Conditions of Polysaccharide from Ledum palustre L. Using Response Surface Analysis

作　　者：张乔会[1] 逄锦慧 杨喆[1] 王建中[1] 

机构地区：[1]北京林业大学北京林业大学林业食品加工与安全北京市重点实验室,北京100083

出　　处：《天然产物研究与开发》2015年第6期1105-1111,共7页Natural Product Research and Development

基　　金：林业公益性行业科研专项(201004081)

摘　　要：杜香是中国东北广泛分布的一种蕴藏量大的植物资源,学者对其成分做了很多的研究,但对杜香多糖的研究极少,本文以杜香为原料,采取水提醇沉法提取多糖,在单因素实验基础上选取实验因素与水平,根据Box-Behnken中心组合实验设计原理采用四因素三水平的响应面分析法,得到优化提取工艺为:提取温度为80℃,提取时间为6 h,料液比为1∶35(m/v),物料粒度为80目,在此条件下,多糖提取得率可达4.19%。利用红外和紫外分析,提取物含多糖典型基团,粗提物中多糖含量为92.6%。Ledum palustre L.,is a kind of source plant widely distributed in the northeast of China. In this study,watersoluble polysaccharide extracted from L. palustre with high extraction yield was successfully studied by the method of water extraction and ethanol deposition. To improve the extraction yield of the Ledum polysaccharides,a Box-Behnken design was employed based on four factor experiments. The four independent variables,namely extraction temperature,particle size,solid-liquid ratio and extraction time on extraction yield of polysaccharides were evaluated by response surface methodology（ RSM）. The results showed that the optimum conditions for extracting Ledum polysaccharide were as follows： extraction temperature 80 ℃,particle size of Ledum 0. 178 mm,solid-liquid ratio 1∶ 35,and extraction time 6h. Under the optimal conditions,the extraction yield achieved 4. 19%. Furthermore,FT-IR and UV analyses showed that L.palustre extract contained typical group of polysaccharide,the content of polysaccharide in the crude extract of L. palustre was determined to be 92. 6%.

关 键 词：杜香 响应面 多糖 提取 

分 类 号：TS241[轻工技术与工程—制糖工程]