正交法优化普通油茶花粉萌发的培养成分  

The Optimization of Medium Compositions of the Pollen Germination of Camellia oleifera by Orthogonal Method

作　　者：黄国文[1] 管天球[1] 赵雨云[1] 陈莫林[1] 刘宏辉[1] 

机构地区：[1]湖南科技学院生化系,湖南永州425199

出　　处：《安徽农业科学》2015年第19期150-151,231,共3页Journal of Anhui Agricultural Sciences

基　　金：湖南省科技计划重点项目(2014NK2021)

摘　　要：[目的]研究普通油茶离体花粉的培养条件。[方法]以蔗糖、维生素C、H3BO4、Mg SO4、培养液p H为影响油茶花粉萌发的因素,进行单因素试验和正交试验,研究这些成分对油茶花粉萌发的影响。[结果]油茶花粉萌发的最佳培养液成分为蔗糖150 g/L,维生素C0.02 g/L,H3BO40.15 g/L,Mg SO40.07 g/L,培养基p H 6;影响油茶花粉萌发主要的因素是蔗糖,其次是H3BO4。[结论]该研究结果为油茶的有性杂交打下了基础。[Objective] This paper researched the cultivation conditions of vitro pollen of Camellia oleifera. [Method]The single factor experiments and the orthogonal experiments on the liquid medium p H and elements（ sucrose,vitamine C,boric acid and magnesium sulfate） were carried out. The effects of these nutrient elements on the pollen germination rate of Camellia oleifera were studied. The results showed that the optimal medium elements of pollen germination from Camellia oleifera were as follow： sucrose 150 g / L,vitamine C 0. 02 g / L,boric acid 0. 15 g / L,magnesium sulfate 0. 07 g / L and p H 6. The main effect factor of pollen germinations was sugar,and follow by boric acid. [Conclusion]The results of this study will lay a foundation for sexual hybridization of Camellia oleifera.

关 键 词：油茶 花粉萌发 培养液成分 正交法 

分 类 号：S794.4[农业科学—林木遗传育种]