荧光分光光度法测定水产品中的石油烃污染物  

Rapid Determination of Petroleum Hydrocarbon Contaminants in Aquatic Products by Fluorescence Spectrophotometry

作　　者：薄尔琳 郭楠 罗靳 郑怀东 刘学光 

机构地区：[1]辽宁省水产品质量安全检验检测局 [2]辽宁省水产技术推广总站,辽宁沈阳110031

出　　处：《辽宁农业科学》2015年第3期27-30,共4页Liaoning Agricultural Sciences

基　　金：辽宁省海洋与渔业厅项目(201201)

摘　　要：建立了检测水产品中石油烃残留的分析方法。样品与过量的碱液在无水乙醇环境下，40℃水浴避光振摇5 h，再经二氯甲烷萃取、负压蒸干、脱芳石油醚溶解，荧光分光光度法测定。结果表明，油标液在0．0～10．0μg ／ml 线性关系良好，相关系数0．9996，检出限为0．85 mg ／kg，添加质量浓度5．0～20．0 mg ／kg 时，样品回收率在91．5％～97．9％之间。该方法与目前我国海洋生物中石油烃的检测方法 GB17378．6-2007相比，节省时间，且皂化充分，检测结果准确度好，精密度高，适用于水产品中石油烃含量的检测。Determination of fluorescence spectrophotometry is established analysis methods of petroleum hydro-carbon residues in aquatic products.Samples and excessive alkali liquor under the environment of anhydrous ethanol,40 ℃ water bath avoid light vibration wave five hours,fully by the methylene chloride extraction after saponification,negative pressure dry,aromatic petroleum ether dissolved,fluorescence spectrophotometry.Re-sults show that the oil standard fluid in 0.0 ~ 10.0 ng /ml linear relationship is good,the correlation coeffi-cient of 0.9996,the detection limit of 0.85 mg /kg,added mass concentration is 5.0 ~20.0 mg /kg,recov-ery is between 91 .5% ~97.9%.Compare to GB17378.6-2007,the method saves time,saponification,ac-curacy of test results is good,high precision,suitable for detection of petroleum hydrocarbon content in aquat-ic products.

关 键 词：检测 荧光分光光度法 水产品 石油烃 皂化 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]