尿脱落细胞染色体畸变与膀胱癌组织学关系的研究  

Relationship between chromosomal aberrations of urine exfoliated cells and histological findings of bladder cancer

作　　者：冉俊武[1] 吕军[2] 王尉[2] 曾钦松[2] 陈晓东[2] 胡卫列[2] 王伟[1] 

机构地区：[1]柳州市人民医院泌尿外科,广西545000 [2]广州军区广州总医院泌尿外科,全军泌尿外科中心,510010

出　　处：《实用医学杂志》2015年第12期1925-1928,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金面上项目(编号:No.81372744);国家自然科学基金青年科学基金资助项目(编号:81001142)

摘　　要：目的:探讨荧光原位杂交(FISH)检测尿脱落细胞常见染色体畸变与膀胱癌病理分级、分期的关系。方法:99例患者尿液标本采用FISH技术检测9p21位点及3、7、17号染色体的畸变,并收集术后肿瘤分级、分期信息。结果:(1)3、17号染色体的畸变率在不同分期、分级间差异均有统计学意义(P<0.05),7号染色体的畸变率在不同分级间差异有统计学意义(P<0.05),在不同病理分期间差异无统计学意义(P>0.05)。9p21位点的畸变率在不同病理分期、分级间差异均无统计学意义(P>0.05)。(2)病理分级、分期越高,3、7、17号染色体扩增倍数越高。分级、分期越低,9p21出现缺失几率越大;分级、分期升高,9p21出现扩增几率越大。结论:4种染色体畸变尤其是扩增与膀胱癌恶性度、肌层浸润密切相关,多倍体数目可能成为膀胱癌预后预测的一个生物学指标。Objective To detect the relationship of pathological grade and stage of bladder cancer with common chromosomal aberrations of urine exfoliated cells by FISH. Methods A total of 99 urine samples were detected by FISH with probes of chromosomes 3,7,17 and 9p21 to collect pathological grade and stage information of bladder tumor tissues. Results （1） The aberrations of chromosome 3 and 17 had significant correlation with pathological grade and stage （P〈0.05） but that of chromosome 7 had no correlation with pathological stage （P〉0.05）, but had correlation with grade （P 〈 0.05）. The aberration of 9p21 had no correlations with pathological grade or stage （P 〉0.05）. （2）The polysomic chromosomal aberrations of chromosomes 3, 7 and 17 assessed correlated with high-grade and high-stage bladder carcinoma. The 9p21 deletion was found at a significant frequency in low-grade and low-stage lesions, when, 9p21 amplification was found at a significant frequency in high-grade and high-stage lesions. Conclusions Aberrations of the four chromosomes, especially polysomic chromosomal aberrations of the bladder cancer cases could present a possible trend toward greater chromosome increased with tumor grades and progressive stages of invasion.

关 键 词：膀胱肿瘤 荧光原位杂交 尿脱落细胞 染色体畸变 

分 类 号：R737.14[医药卫生—肿瘤]